Application Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
Rat anti-Mouse CD11b Monoclonal Antibody | anti-CD11b antibody
Anti-Mouse CD11b, No Azide (Clone M1/70.15)(rat IgG2b)
Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
CD11b, Antibody; Anti-Mouse CD11b, No Azide (Clone M1/70.15)(rat IgG2b); anti-CD11b antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
M1/70.15
Specificity
Mouse CD11b (Mac-1; Ly 40; CR3a)
Purity/Purification
Purified
Form/Format
No Azide. Purified Ig buffered in PBS, no preservative, 0.2um sterile filtered.
Applicable Applications for anti-CD11b antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
Conjugation
Unconjugated
Preparation and Storage
For long term storage(Purified, Biotin, FITC, No Azide), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Application Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
Application Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
FCM/FACS (Flow Cytometry)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
FCM/FACS (Flow Cytometry)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
Related Product Information for anti-CD11b antibody
anti-mouse CD11b (Mac-1; Ly 40) monoclonal antibody detects the 170kDa alpha subunit of Mac-1 which mediates adhesion to ICAM-1 (CD54) and C3bi. Mac-1 is expressed on granulocytes, macrophages, natural killer cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 is up-regulated on neutrophils after activation. This particular clone blocks cell adherence and C3bi binding but does not block cell mediated lysis (1,2,3,4,5,6).Applications include flow cytometry, in vitro and in vivo blocking, immunohistochemistry for both paraffin embedded and acetone-fixed frozen sections 1-20ug/ml (7). Also useful for immunoprecipitation and western blotting.
References
1. Sanchez-Madrid, F., P. Simon, S. Thompson, et al. 1983. Mapping of antigenic and functional epitopes on the alpha and beta subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J. Exp. Med. 158:586-602.
2. Springer, T., G. Galfre, D.S. Secher, et al. 1978 Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539 - 551.
3. Springer, T., G. Galfre, D.S. Secher, et al. 1979. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur. J. Immunol. 9:301 - 306.
4. Vignali, D.A., et al. 1990. Antibody-Dependent killing of Schistosoma mansoni Schistosomula in vitro by Starch-elicited murine macrophages. J. Immunol. 144: 4030 -40378.
5. Kantor, A., et al. 1992. Differential development of progenitor activity for three B-cell lineages. Proc. Natl. Acad. Sci. U.S.A. 89: 3320 - 3324.
6. Jutial, M.A., et al. 1988. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma. J. Immunol. 18: 1819 - 1826.
7. Whiteland, J.L. et al. (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. Volume 43, pp 313-320.
2. Springer, T., G. Galfre, D.S. Secher, et al. 1978 Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539 - 551.
3. Springer, T., G. Galfre, D.S. Secher, et al. 1979. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur. J. Immunol. 9:301 - 306.
4. Vignali, D.A., et al. 1990. Antibody-Dependent killing of Schistosoma mansoni Schistosomula in vitro by Starch-elicited murine macrophages. J. Immunol. 144: 4030 -40378.
5. Kantor, A., et al. 1992. Differential development of progenitor activity for three B-cell lineages. Proc. Natl. Acad. Sci. U.S.A. 89: 3320 - 3324.
6. Jutial, M.A., et al. 1988. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma. J. Immunol. 18: 1819 - 1826.
7. Whiteland, J.L. et al. (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. Volume 43, pp 313-320.
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Product Notes
The CD11b (Catalog #AAA74298) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD11b, No Azide (Clone M1/70.15)(rat IgG2b) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD11b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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