anti-Human CD11b/C3 Monoclonal Antibody
Mouse anti Human CD11b/C3
Reactivity
Human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
CD11b/C3, Antibody; Mouse anti Human CD11b/C3; anti-CD11b/C3 antibody
Host
Mouse (Balb/c)
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Bear-1
Specificity
Bear-1 reacts with the CD11b alpha chain of the CD11b/CD18. Bear-1 precipitated a 165-95-kDa complex and is found to be similar to Mo-1 (monocyte differentiation antigen).
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-CD11b/C3 antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
Source Note
Bear-1 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with with purified Human monocytes.
Preparation and Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Related Product Information for anti-CD11b/C3 antibody
Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (alphaMbeta2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). ITGAM is also known as CR3A, and cluster of differentiation molecule 11B (CD11B). The second chain of alphaMbeta2 is the common integrin beta2 subunit known as CD18, and integrin alphaMbeta2 thus belongs to the beta2 subfamily (or leukocyte) integrins. alphaMbeta2 is expressed on the surface of many leukocytes involved in the innate immune system, including monocytes, granulocytes, macrophages, and natural killer cells. It mediates inflammation by regulating leukocyte adhesion and migRation and has been impliCated in several immune processes such as phagocytosis, cell-mediated cytotoxicity, chemotaxis and cellular activation. It is involved in the complement system due to its capacity to bind inactivated complement component 3b (iC3b).
Product Categories/Family for anti-CD11b/C3 antibody
References
G.D. Keizer, J. Borst, C.G. Figdor, H. Spits, F. Miedema, C. Terhorst and J.E. de Vries.Biochemical and functional characteristics of the Human leukocyte membrane antigen family LFA-1, Mo-1 and p150,95. Eur. J. Immunol. 15: 1142-1147, 1985.
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Product Notes
The CD11b/C3 (Catalog #AAA77500) is an Antibody produced from Mouse (Balb/c) and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human CD11b/C3 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b/C3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD11b/C3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b/C3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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