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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human CD11b/MAC-1 Monoclonal Antibody | anti-CD11b/MAC-1 antibody
CD11b/MAC-1 (Microglial Marker)
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CD11b/MAC-1, Antibody; CD11b/MAC-1 (Microglial Marker); CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; Leukocyte adhesion receptor MO1; Ly-40; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit; anti-CD11b/MAC-1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
ITGAM/3338
Specificity
CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin aM combines with the Integrin '2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin aM/'2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b andCD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
1153
Applicable Applications for anti-CD11b/MAC-1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Positive Control
Monocytes & granulocytes. Lymph nodes, spleen and tonsil (IHC).
Immunogen
Recombinant fragment (around aa941-1074) of human ITGAM protein (exact sequence is proprietary)
Cellular Localization
Cell Surface
Hu-chromosome Location
16p11.2
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).)
IHC (Immunohistochemisry)
(Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).)
IHC (Immunohistochemisry)
(Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).)
SDS-PAGE
(SDS-PAGE Analysis Purified CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338). Confirmation of Purity and Integrity of Antibody.)
WB (Western Blot)
(Western Blot Analysis of spleen tissue lysate using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).)
WB (Western Blot)
(Western Blot Analysis of spleen tissue lysate using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).)
References
Springer T, et al. 1978. Eur. J. Immunol. 8:539
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95kDa & 170kDa
NCBI Official Full Name
integrin alpha-M isoform 1
NCBI Official Synonym Full Names
integrin subunit alpha M
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
NCBI Protein Information
integrin alpha-M
UniProt Protein Name
Integrin alpha-M
UniProt Gene Name
ITGAM
UniProt Synonym Gene Names
CD11B; CR3A
UniProt Entry Name
ITAM_HUMAN
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Product Notes
The CD11b/MAC-1 itgam (Catalog #AAA215041) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD11b/MAC-1 (Microglial Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b/MAC-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD11b/MAC-1 itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b/MAC-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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