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WB (Western Blot)
(Detection of human CD11b by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Jurkat, KG-1, Hep-G2, THP-1, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
CD11b recombinant antibody
Rabbit anti-CD11b Recombinant Monoclonal Antibody [BLR107H]
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CD11b, Recombinant Antibody; Rabbit anti-CD11b Recombinant Monoclonal Antibody [BLR107H]; integrin, alpha M (complement component 3 receptor 3 subunit); Neutrophil adherence receptor; MO1A; macrophage antigen alpha polypeptide; MAC1A; MAC-1; leukocyte adhesion receptor MO1; neutrophil adherence receptor alpha-M subunit; SLEB6; integrin alpha-M; CD antigen CD11b; CD11 antigen-like family member B; CD11B; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; antigen CD11b (p170); Integrin alpha-M; integrin subunit alpha M; CD11b recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR107H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for CD11b recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 17-334 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD11b by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Jurkat, KG-1, Hep-G2, THP-1, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human CD11b by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Jurkat, KG-1, Hep-G2, THP-1, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human CD11b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MUTZ-3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1) used for IP at 20 ul/mg lysate. CD11b was also immunoprecipitated by rabbit anti-CD11b antibody BL-1559A-1H9. For blotting immunoprecipitated CD11b, AAA213581 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CD11b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MUTZ-3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1) used for IP at 20 ul/mg lysate. CD11b was also immunoprecipitated by rabbit anti-CD11b antibody BL-1559A-1H9. For blotting immunoprecipitated CD11b, AAA213581 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry)
(Detection of human CD11b in FFPE lung by IHC. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human CD11b in FFPE lung by IHC. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human CD11b in FFPE spleen by IHC. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human CD11b in FFPE spleen by IHC. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD11b in FFPE MUTZ-3 cells by ICC. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD11b in FFPE MUTZ-3 cells by ICC. Antibody: Rabbit anti-CD11b recombinant monoclonal antibody (AAA213581 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for CD11b recombinant antibody
CD11b is the most commonly used name for Integrin alpha M (ITGAM). Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAM combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). ITGAM/ITGB2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. [taken from NCBI Entrez Gene (Gene ID: 3684)].
Product Categories/Family for CD11b recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
127,179 Da
NCBI Official Full Name
integrin alpha-M isoform 1
NCBI Official Synonym Full Names
integrin, alpha M (complement component 3 receptor 3 subunit)
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
NCBI Protein Information
integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subuni
UniProt Protein Name
Integrin alpha-M
UniProt Gene Name
ITGAM
UniProt Synonym Gene Names
CD11B; CR3A
UniProt Entry Name
ITAM_HUMAN
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Product Notes
The CD11b itgam (Catalog #AAA213581) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD11b Recombinant Monoclonal Antibody [BLR107H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD11b itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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