Mouse CD127/IL7R Monoclonal Antibody | anti-IL7R antibody

CD127/IL7R (Marker of T-Reg Cells)

Cat. Num. AAA214766

• Gene Names: IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
• Reactivity: Human. Others not known.
• Synonyms: CD127/IL7R, Antibody; CD127/IL7R (Marker of T-Reg Cells); CD127; IL-7 receptor subunit alpha; IL-7R-alpha; IL7R; IL7RA; IL7R alpha; ILRA; Interleukin 7 receptor alpha chain; Interleukin 7 receptor; anti-IL7R antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone Number: IL7R/2751
• Specificity: The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 298

• Immunogen: Recombinant fragment of human CD127 protein (IL7R) (around aa 21-236) (exact sequence is proprietary)
• Positive Control: K562, HeLa, Daudi or Jurkat Cells. Tonsil, Lymph Node or Spleen
• Cellular Localization: Cell Surface and Cytoplasmic
• Chromosome Location: 5p13.2
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS_PAGE

(SDS-PAGE Analysis Purified CD127 Mouse Monoclonal Antibody (IL7R/2751). Confirmation of Integrity and Purity of Antibody.)

Application Data

(Analysis of Protein Array containing >19,000 full-length human proteins using CD127 Mouse Monoclonal Antibody (IL7R/2751). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified CD127 Mouse Monoclonal Antibody (IL7R/2751). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot)

(Western Blot Analysis of Human K562, Jurkat, HeLa and Ramos cell lysate using CD127 Mouse Monoclonal Antibody (IL7R/2751).)

References

Weihong Liu et. al. CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells. J Exp Med. 2006 Jul 10; 203(7): 1701-1711.

NCBI and Uniprot Product Information

• NCBI GI #: 28610151
• NCBI GeneID: 3575
• NCBI Accession #: NP_002176.2
• NCBI GenBank Nucleotide #: NM_002185.4
• UniProt Accession #: P16871; Q05CU8; Q6NSP4; Q6NWM0; Q6NWM1; Q6NWM2; Q6NWM3; Q6SV45; Q9UPC1; B2RCS6; B4DVT1
• Molecular Weight: 52-72kDa
• NCBI Official Full Name: interleukin-7 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 7 receptor
• NCBI Official Symbol: IL7R
• NCBI Official Synonym Symbols: ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
• NCBI Protein Information: interleukin-7 receptor subunit alpha
• UniProt Protein Name: Interleukin-7 receptor subunit alpha
• UniProt Gene Name: IL7R
• UniProt Synonym Gene Names: IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA