Mouse CD13 Monoclonal Antibody | anti-CD13 antibody

Anti-Hu CD13 PE-Cy7

Cat. Num. AAA129170

• Gene Names: ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
• Reactivity: Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with tandem dye PE-Cy7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD13, Antibody; Anti-Hu CD13 PE-Cy7; Aminopeptidase N; APN; PEPN; ANPEP; gp150; LAP1; alanyl aminopeptidase, membrane; anti-CD13 antibody

• Host: Mouse
• Reactivity: Human, Non-human primates
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: WM15
• Specificity: The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
• Purity/Purification: The purified antibody is conjugated with tandem dye PE-Cy7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD13 antibody: FCM/FACS (Flow Cytometry)
• Conjugation: PE-Cy7
• Immunogen: Human AML cells
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Related Product Information for anti-CD13 antibody

CD13 (aminopeptidase N, APN) is a 150kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

References

*Favaloro EJ, Browning T, Facey D: CD13 (GP150; aminopeptidase-N): predominant Functional Application (FUNC)tional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec;21(13):1695-701., URL: https://www.ncbi.nlm.nih.gov/pubmed/7902291dopt=A,*McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555., URL: https://www.ncbi.nlm.nih.gov/pubmed/23243270,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL:,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL:,*Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987)., URL:,*Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep;61(1):11-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/4052321dopt=A,*Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul;17(3):392-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/3865147dopt=A
*Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 1;12(13):3971-8., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1;110(1):142-50., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27;7:140., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=

NCBI and Uniprot Product Information

• NCBI GI #: 157266300
• NCBI GeneID: 290
• NCBI Accession #: NP_001141.2
• NCBI GenBank Nucleotide #: NM_001150.2
• UniProt Accession #: P15144; Q16728; Q6GT90; Q8IUK3; Q8IVH3; Q9UCE0
• Molecular Weight: 109,540 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl (membrane) aminopeptidase
• NCBI Official Symbol: ANPEP
• NCBI Official Synonym Symbols: APN; CD13; LAP1; P150; PEPN; GP150
• NCBI Protein Information: aminopeptidase N; AP-M; AP-N; hAPN; aminopeptidase M; alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13
• UniProt Protein Name: Aminopeptidase N
• UniProt Gene Name: ANPEP
• UniProt Synonym Gene Names: APN; CD13; PEPN; AP-N; hAPN; AP-M
• UniProt Entry Name: AMPN_HUMAN