IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-CD134antibodyOX-40(AAA72444) Immunofluorescenceanalysisofparaformaldehydefixedrat(Rattusnorvegicus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofOX-40(AAA72444)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingpunctatecytoplasmicstaining,possiblyofvesiclestraffickingprotein.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72444,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
CD134 recombinant antibody
Anti-CD134 [OX-40], Rabbit IgG, kappa
Gene Names
Tnfrsf4; Ox40; Txgp1
Reactivity
Rat
Applications
Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Functional Assay, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD134, Recombinant Antibody; Anti-CD134 [OX-40], Rabbit IgG, kappa; CD134; Anti-CD134 [OX-40]; TNFRS4; Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; OX40; MRC OX40; MRC OX-40; OX-40; CD134 recombinant antibody
Host
Rabbit
Reactivity
Rat
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
OX-40
Specificity
OX-40 antibody binds specifically to CD4+ T blasts. CD134 is a protein of the TNFR superfamily. It binds to OX40 ligand of both the monomeric and trimeric states which results in co-stimulated proliferation.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD134 recombinant antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), FA (Functional Assay), FCM/FACS (Flow Cytometry)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-CD134antibodyOX-40(AAA72444) Immunofluorescenceanalysisofparaformaldehydefixedrat(Rattusnorvegicus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofOX-40(AAA72444)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingpunctatecytoplasmicstaining,possiblyofvesiclestraffickingprotein.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72444,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-CD134antibodyOX-40(AAA72444) Immunofluorescenceanalysisofparaformaldehydefixedrat(Rattusnorvegicus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofOX-40(AAA72444)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingpunctatecytoplasmicstaining,possiblyofvesiclestraffickingprotein.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72444,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusinganti-CD134(Tumornecrosisfactorreceptorsuperfamilymember4)antibodyOX-40(AAA72444) Stainingofunstimulated(blue)and3-dayConcanavalinAstimulated(red)rat(Rattusnorvegicus)lymphocyteswiththerabbit-chimericversionofOX-40(AAA72444)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusingaAlexaFluor488conjugatedsecondaryanti-rabbitantibody.ConcanavalinAstimulationinducesCD134expressionasevidentbytheincreasedfluorescencesignal.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusinganti-CD134(Tumornecrosisfactorreceptorsuperfamilymember4)antibodyOX-40(AAA72444) Stainingofunstimulated(blue)and3-dayConcanavalinAstimulated(red)rat(Rattusnorvegicus)lymphocyteswiththerabbit-chimericversionofOX-40(AAA72444)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusingaAlexaFluor488conjugatedsecondaryanti-rabbitantibody.ConcanavalinAstimulationinducesCD134expressionasevidentbytheincreasedfluorescencesignal.)
Related Product Information for CD134 recombinant antibody
OX40 antibody was prepared by immunizing mice with phytohaemagglutinin-activated rat lymph node cells.
References
Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987 Dec;24(12):1281-90. N/A2828930
Note on publication:
Describes the identification of and functional studies using antibodies which can act as markers for activated T cells.
Note on publication:
Describes the identification of and functional studies using antibodies which can act as markers for activated T cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
29,895 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 4
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 4
NCBI Official Symbol
Tnfrsf4
NCBI Official Synonym Symbols
Ox40; Txgp1
NCBI Protein Information
tumor necrosis factor receptor superfamily member 4; MRC OX40; OX40 antigen; OX40L receptor; tumor necrosis factor superfamily, member 4; Tax-transcriptionally activated glycoprotein 1; tumor necrosis factor (ligand) superfamily member 4
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 4
UniProt Gene Name
Tnfrsf4
UniProt Synonym Gene Names
Ox40; Txgp1l
UniProt Entry Name
TNR4_RAT
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Product Notes
The CD134 tnfrsf4 (Catalog #AAA72444) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD134 [OX-40], Rabbit IgG, kappa reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD134 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), FA (Functional Assay), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD134 tnfrsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD134, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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