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product-image-AAA23920_APP7.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Mouse anti-Human CD137/4-1BB/TNFRSF9 Monoclonal Antibody | anti-TNFRSF9 antibody

CD137/4-1BB/TNFRSF9

Gene Names
TNFRSF9; ILA; 4-1BB; CD137; CDw137
Reactivity
Human
Applications
ELISA, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CD137/4-1BB/TNFRSF9, Antibody; CD137/4-1BB/TNFRSF9; 4-1BB Ligand Receptor T Cell; Antigen 4-1BB Homolog; CDw137; HLDA VI; Homolog of Mouse 4 1BB; induced by lymphocyte activation (ILA); Interleukin activated receptor homolog of Mouse Ly63; T-cell antigen 4-1BB/ILA homolog; Tumor necrosis factor receptor superfamily member 9; anti-TNFRSF9 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
4-1BB/3201
Specificity
CD137 belongs to the tumor necrosis factor receptor family and delivers a costimulatory signal to T lymphocytes. It is expressed on activated T cells and binds an inducible ligand that is found on B cells, macrophages and dendritic cells. Interactions between CD137 and its ligand are involved in antigen presentation and the generation of cytotoxic T cells. CD137 antibody may improve cancer treatment, and has been implicated in breast cancer, melanoma and lymphoma.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
255
Applicable Applications for anti-TNFRSF9 antibody
ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin
Application Notes
ELISA: For coating, order antibody without BSA
IF: 1-2ug/ml
FC/FACS: 1-2ug/million cells
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
HEK293 cells, Jurkat whole cell lysate or recombinant human CD137 protein (WB). Human kidney cancer, liver, stomach or thyroid cancer tissue (IHC-P). Expressed on the surface of activated T- cells.
Immunogen
A recombinant fragment (around aa 19-188) of human CD137/4-1BB/TNFRSF9 protein (exact sequence is proprietary)
Cellular Localization
Cell Surface
Hu-chromosome Location
1p36.23
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

product-image-AAA23920_APP7.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IF (Immunofluorescence)

(Immunofluorescent staining of MeOH-fixed HEK293 cells. CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (red).)

product-image-AAA23920_IF6.jpg IF (Immunofluorescence) (Immunofluorescent staining of MeOH-fixed HEK293 cells. CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (red).)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of MeOH-fixed HEK293 cells. CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

product-image-AAA23920_FCM5.jpg FCM (Flow Cytometry) (Flow Cytometric Analysis of MeOH-fixed HEK293 cells. CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Cerebellum stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)

product-image-AAA23920_IHC4.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Cerebellum stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Spleen stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)

product-image-AAA23920_IHC3.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Spleen stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)

product-image-AAA23920_IHC2.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)

product-image-AAA23920_IHC.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)
References
Zhao S, et al. CD137 ligand is expressed in primary and secondary lymphoid follicles and in B-cell lymphomas: diagnostic and therapeutic implications. Am J Surg Pathol. 2013 Feb;37(2):250-8.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32kDa (monomer); 85kDa (dimer)
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 9
NCBI Official Synonym Full Names
TNF receptor superfamily member 9
NCBI Official Symbol
TNFRSF9
NCBI Official Synonym Symbols
ILA; 4-1BB; CD137; CDw137
NCBI Protein Information
tumor necrosis factor receptor superfamily member 9
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 9
UniProt Gene Name
TNFRSF9
UniProt Synonym Gene Names
CD137; ILA
UniProt Entry Name
TNR9_HUMAN

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Product Notes

The TNFRSF9 tnfrsf9 (Catalog #AAA23920) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD137/4-1BB/TNFRSF9 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD137/4-1BB/TNFRSF9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin. ELISA: For coating, order antibody without BSA IF: 1-2ug/ml FC/FACS: 1-2ug/million cells Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the TNFRSF9 tnfrsf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD137/4-1BB/TNFRSF9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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