CD137 Recombinant Antibody | TNFRSF9 recombinant antibody

Rabbit anti-CD137 Recombinant Monoclonal Antibody [BLR051F]

Cat. Num. AAA213549

• Gene Names: TNFRSF9; ILA; 4-1BB; CD137; CDw137
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: CD137, Recombinant Antibody; Rabbit anti-CD137 Recombinant Monoclonal Antibody [BLR051F]; ILA; interleukin-activated receptor, homolog of mouse Ly63; tumor necrosis factor receptor superfamily member 9; T-cell antigen ILA; T-cell antigen 4-1BB homolog; receptor protein 4-1BB; induced by lymphocyte activation (ILA); CDw137; 4-1BB; CD137 antigen; T cell antigen ILA; CD137; CD antigen CD137; 4-1BB ligand receptor; homolog of mouse 4-1BB; Tumor necrosis factor receptor superfamily member 9; TNF receptor superfamily member 9; TNFRSF9 recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: BLR051F
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for TNFRSF9 recombinant antibody: FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Residues 24-189 (ECD)
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human CD137 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, HDLM-2, My-La CD4+, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (AAA213549 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation)

(Detection of human CD137 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD137 recombinant monoclonal antibody (AAA213549 lot 4) used for IP at 6 ul/mg lysate. CD137 was also immunoprecipitated by a previous lot of this antibody (AAA213549 lot 3). For blotting immunoprecipitated CD137, AAA213549 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemisry)

(Detection of human CD137 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (AAA213549). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human CD137 in FFPE HDLM-2 by ICC. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (AAA213549). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM/FACS (Flow Cytometry)

(Detection of human CD137 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (AAA213549) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for TNFRSF9 recombinant antibody

CD137 is a member of the TNF-receptor superfamily; it is a receptor that contributes to the clonal expansion, survival, and development of T cells. CD137 can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. [provided by RefSeq, Jul 2008] [taken from NCBI Entrez Gene (Gene ID: 3604)].

Product Categories/Family for TNFRSF9 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5730095
• NCBI GeneID: 3604
• NCBI Accession #: NP_001552.2
• NCBI GenBank Nucleotide #: NM_001561.5
• UniProt Accession #: Q07011; AAB34924
• Molecular Weight: 27,899 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 9
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 9
• NCBI Official Symbol: TNFRSF9
• NCBI Official Synonym Symbols: ILA; 4-1BB; CD137; CDw137
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 9; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interl
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 9
• UniProt Gene Name: TNFRSF9
• UniProt Synonym Gene Names: CD137; ILA
• UniProt Entry Name: TNR9_HUMAN