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FCM (Flow Cytometry)
(Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 ul/Test,left) or CD141/Thrombomodulin Rabbit PolymAb®(AAA28558,2 ug/mL,right).)
Rabbit anti-Human CD141/Thrombomodulin Monoclonal Antibody | anti-THBD antibody
CD141/Thrombomodulin Rabbit PolymAb
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Affinity purification
Synonyms
CD141/Thrombomodulin, Antibody; CD141/Thrombomodulin Rabbit PolymAb; TM; THRM; AHUS6; BDCA3; CD141; BDCA-3; THPH12; anti-THBD antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
APAEPQPGGSQCVEHDCFALYPGPATFLNASQICDGLRGHLMTVRSSVAADVISLLLNGDGGVGRRRLWIGLQLPPGCGDPKRLGPLRGFQWVTGDNNTSYSRWARLDLNGAPLCGPLCVAVSAAEATVPSEPIWEEQQCEVKADGFLCEFHFPATCRPLAVEPGAAAAAVSITYGTPFAARGADFQALPVGSSAAVAPLGLQLMCTAPPGAVQGHWAREAPGAWDCSVENGGCEHACNAIPGAPRCQCPAGAALQADGRSCTASATQSCNDLCEHFCVPNPDQPGSYSCMCETGYRLAADQHRCEDVDDCILEPSPCPQRCVNTQGGFECHCYPNYDLVDGECVEPVDPCFRANCEYQCQPLNQTSYLCVCAEGFAPIPHEPHRCQMFCNQTACPADCDPNTQASCECPEGYILDDGFICTDIDECENGGFCSGVCHNLPGTFECICGPDSALARHIGTDCDSGKVDGGDSGSGEPPPSPTPGSTLTPPAVGLVHS
Applicable Applications for anti-THBD antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:2000-1:10000
IHC-P: 1:100-1:500
IF/ICC: 1:50-1:200
FC: 1:500-1:1000
ELISA: Recommended starting concentration is 1ug/mL.
Please optimize the concentration based on your specific assay requirements.
IHC-P: 1:100-1:500
IF/ICC: 1:50-1:200
FC: 1:500-1:1000
ELISA: Recommended starting concentration is 1ug/mL.
Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-515 of human CD141/Thrombomodulin. (NP_000352.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
FCM (Flow Cytometry)
(Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 ul/Test,left) or CD141/Thrombomodulin Rabbit PolymAb®(AAA28558,2 ug/mL,right).)
FCM (Flow Cytometry)
(Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 ul/Test,left) or CD141/Thrombomodulin Rabbit PolymAb®(AAA28558,2 ug/mL,right).)
FCM (Flow Cytometry)
(Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and THP-1 cells (right) were surface-stained with CD141/Thrombomodulin Rabbit PolymAb® (AAA28558,2 ug/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 ul/Test,blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).)
FCM (Flow Cytometry)
(Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and THP-1 cells (right) were surface-stained with CD141/Thrombomodulin Rabbit PolymAb® (AAA28558,2 ug/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 ul/Test,blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).)
IF (Immunofluorescence)
(Immunofluorescence analysis of THP-1 cells using CD141/Thrombomodulin Rabbit PolymAb® (AAA28558) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of THP-1 cells using CD141/Thrombomodulin Rabbit PolymAb® (AAA28558) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A431 cells using CD141/Thrombomodulin Rabbit PolymAb® (AAA28558) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A431 cells using CD141/Thrombomodulin Rabbit PolymAb® (AAA28558) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using CD141/Thrombomodulin Rabbit PolymAb® (AAA28558) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using CD141/Thrombomodulin Rabbit PolymAb® (AAA28558) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot)
(Western blot analysis of lysates from A-431 cells, using CD141/Thrombomodulin Rabbit PolymAb® (AAA28558) at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of lysates from A-431 cells, using CD141/Thrombomodulin Rabbit PolymAb® (AAA28558) at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Related Product Information for anti-THBD antibody
The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia.
Product Categories/Family for anti-THBD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 60kDa
Observed MW: 90kDa
Observed MW: 90kDa
UniProt Protein Name
Thrombomodulin
UniProt Gene Name
THBD
UniProt Synonym Gene Names
THRM; TM
UniProt Entry Name
TRBM_HUMAN
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Product Notes
The THBD thbd (Catalog #AAA28558) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD141/Thrombomodulin Rabbit PolymAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD141/Thrombomodulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:2000-1:10000 IHC-P: 1:100-1:500 IF/ICC: 1:50-1:200 FC: 1:500-1:1000 ELISA: Recommended starting concentration is 1ug/mL. Please optimize the concentration based on your specific assay requirements. Researchers should empirically determine the suitability of the THBD thbd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: APAEPQPGGS QCVEHDCFAL YPGPATFLNA SQICDGLRGH LMTVRSSVAA DVISLLLNGD GGVGRRRLWI GLQLPPGCGD PKRLGPLRGF QWVTGDNNTS YSRWARLDLN GAPLCGPLCV AVSAAEATVP SEPIWEEQQC EVKADGFLCE FHFPATCRPL AVEPGAAAAA VSITYGTPFA ARGADFQALP VGSSAAVAPL GLQLMCTAPP GAVQGHWARE APGAWDCSVE NGGCEHACNA IPGAPRCQCP AGAALQADGR SCTASATQSC NDLCEHFCVP NPDQPGSYSC MCETGYRLAA DQHRCEDVDD CILEPSPCPQ RCVNTQGGFE CHCYPNYDLV DGECVEPVDP CFRANCEYQC QPLNQTSYLC VCAEGFAPIP HEPHRCQMFC NQTACPADCD PNTQASCECP EGYILDDGFI CTDIDECENG GFCSGVCHNL PGTFECICGP DSALARHIGT DCDSGKVDGG DSGSGEPPPS PTPGSTLTPP AVGLVHS. It is sometimes possible for the material contained within the vial of "CD141/Thrombomodulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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