IP (Immunoprecipitation)
(CD147 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-CD147 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 50 kDa)
Rabbit anti-Human CD147 Monoclonal Antibody | anti-BSG antibody
Recombinant Anti-CD147 Antibody, Rabbit Monoclonal
ELISA: 1:5000-1:10,000
IHC-P: 1:2500-1:10,000
IHC-Fr 1:500-1:2000
ICC/IF: 1:50-1:1000
FC/FACS/FCM: 1:25-1:100
IP: 4-6uL/mg of lysate
IP (Immunoprecipitation)
(CD147 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-CD147 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 50 kDa)
IP (Immunoprecipitation)
(CD147 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-CD147 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 50 kDa)
Application Data
(Analysis of anti-CD147 reactivity on HeLa cells.)
Application Data
(Analysis of anti-CD147 reactivity on HeLa cells.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human esophagus with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human esophagus with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.)
IHC (Immunohistchemistry)
(Immunochemical staining of human CD147 in human liver with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of hepatocyte.)
IHC (Immunohistchemistry)
(Immunochemical staining of human CD147 in human liver with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of hepatocyte.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human placenta with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing positive staining of trophoblast.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human placenta with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing positive staining of trophoblast.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human rectal cancer with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell in intestinal gland.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human rectal cancer with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell in intestinal gland.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human stomach with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD147 in human stomach with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.)
IF (Immunofluorescence)
(Immunofluorescence staining of CD147 in monkey stomach with rabbit monoclonal antibody (1:1000, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI)
IF (Immunofluorescence)
(Immunofluorescence staining of CD147 in monkey stomach with rabbit monoclonal antibody (1:1000, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI)
IF (Immunofluorescence)
(Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells.)
IF (Immunofluorescence)
(Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells.)
NCBI and Uniprot Product Information
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Product Notes
The BSG bsg (Catalog #AAA27726) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD147 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD147 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-Fr), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:2500-1:10,000 IHC-Fr 1:500-1:2000 ICC/IF: 1:50-1:1000 FC/FACS/FCM: 1:25-1:100 IP: 4-6uL/mg of lysate. Researchers should empirically determine the suitability of the BSG bsg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD147, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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