WB (Western Blot)
(Western Blotusinganti-CD147antibodyOX-114(AAA72477) Mouseliverlysate(A),mousebrainlysate(B)andmouseheartlysate(C)samples(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionofOX-114(AAA72477)at2,2and1ug/ml,respectively,beforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforCD147is42.4kDa.)
CD147 recombinant antibody
Anti-CD147 [OX-114], Rat IgG1, kappa
Gene Names
Bsg; HT-7; CD147; EMMPRIN; AI115436; AI325119
Reactivity
Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein A affinity purified
Synonyms
CD147, Recombinant Antibody; Anti-CD147 [OX-114], Rat IgG1, kappa; CD147; Anti-CD147 [OX-114]; Basigin; basic immunoglobulin superfamily; HT7 antigen; membrane glycoprotein gp42; gp42; gp-42; OX114; MRC OX-114; MRC OX114; CD147 recombinant antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
OX-114
Specificity
OX-114 binds to both the 2- and 3-domain isoforms of CD147. CD147 targets monocarboxylate transporters to the plasma membrane, and is involved in spermatogenesis, embryo implantation, neural network fomration and tumor progression. Stimulates production of matrix metalloproteases. Possibly a receptor for oligomannosidic glycans. CD147 found only in vascular endothelium in non-neoplastic regions of the brain whereas it is present in tmour cells but not in proliferating blood vessels in malignant gliomas.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD147 recombinant antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blotusinganti-CD147antibodyOX-114(AAA72477) Mouseliverlysate(A),mousebrainlysate(B)andmouseheartlysate(C)samples(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionofOX-114(AAA72477)at2,2and1ug/ml,respectively,beforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforCD147is42.4kDa.)
WB (Western Blot)
(Western Blotusinganti-CD147antibodyOX-114(AAA72477) Mouseliverlysate(A),mousebrainlysate(B)andmouseheartlysate(C)samples(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionofOX-114(AAA72477)at2,2and1ug/ml,respectively,beforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforCD147is42.4kDa.)
IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedNIH3T3cellsandmousesplenocyteswithanti-CD147antibodyOX-114(AAA72477) ImmunofluorescenceanalysisofparaformaldehydefixedNIH3T3cells(A)andmousesplenocytes(B)permeabilizedwith0.15%TritonandstainedwiththechimericmouseIgG1versionofOX-114(AAA72477)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).PanelsinAandBshowfromleft-right,top-bottomAAA72477,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-unknownspecificityantibodyfollowedbyAlexaFluor 488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedNIH3T3cellsandmousesplenocyteswithanti-CD147antibodyOX-114(AAA72477) ImmunofluorescenceanalysisofparaformaldehydefixedNIH3T3cells(A)andmousesplenocytes(B)permeabilizedwith0.15%TritonandstainedwiththechimericmouseIgG1versionofOX-114(AAA72477)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).PanelsinAandBshowfromleft-right,top-bottomAAA72477,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-unknownspecificityantibodyfollowedbyAlexaFluor 488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-CD147antibodyOX-114(AAA72477) NIH3T3cellswerefixedusing2%PFA,permeabilisedusing0.5%TritonandstainedwithunimmunizedrabbitIgGantibody(MOPC-21;isotypecontrol,blackline)ortherabbitIgG-chimericversionofOX-114(AAA72477,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-CD147antibodyOX-114(AAA72477) NIH3T3cellswerefixedusing2%PFA,permeabilisedusing0.5%TritonandstainedwithunimmunizedrabbitIgGantibody(MOPC-21;isotypecontrol,blackline)ortherabbitIgG-chimericversionofOX-114(AAA72477,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
Related Product Information for CD147 recombinant antibody
OX-114 was generated by immunizing rats with recombinant mCD147d0-2rCD4d3+4.
References
Hanna et al. A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically. BMC Biochem 4:17 2003 N/A14606962
Note on publication:
Describes the original use of this antibody as part of FC and IP studies to identify new markers for activated T cells.
Note on publication:
Describes the original use of this antibody as part of FC and IP studies to identify new markers for activated T cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,674 Da
NCBI Official Full Name
basigin isoform 2
NCBI Official Synonym Full Names
basigin
NCBI Official Symbol
Bsg
NCBI Official Synonym Symbols
HT-7; CD147; EMMPRIN; AI115436; AI325119
NCBI Protein Information
basigin; 5A11/Basigin; HT7 antigen; basic immunoglobulin superfamily; gp 42; membrane glycoprotein gp42; neurothelin
UniProt Protein Name
Basigin
UniProt Gene Name
Bsg
UniProt Entry Name
BASI_MOUSE
Similar Products
Product Notes
The CD147 bsg (Catalog #AAA72477) is a Recombinant Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD147 [OX-114], Rat IgG1, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD147 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD147 bsg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD147, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.