WB (Western Blot)
(Western Blotusinganti-CD147(Basigin)antibodyOX-47(AAA72471)Ratthymustissuelysate(35ugproteininRIPAbuffer)wasresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitversionofOX-47(AAA72471)at0.1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedbandsizeforCD147is42.4kDa.AAA72471successfullydetectedCD147inratthymustissuelysate.)
CD147 recombinant antibody
Anti-CD147 [OX-47], Rabbit IgG, kappa
Gene Names
Bsg; CE9; HT7; 5A11; Ox47R; EMMPRIN; basignin
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, immunoassay, Radioimmunoassay
Purity
Protein A affinity purified
Synonyms
CD147, Recombinant Antibody; Anti-CD147 [OX-47], Rabbit IgG, kappa; CD147; Anti-CD147 [OX-47]; Basigin; BSG; extracellular matrix metalloproteinase inducer; EMMPRIN; Glycoprotein CE9; OX-47 antigen; CD147 recombinant antibody
Host
Rabbit
Reactivity
Rat
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
OX-47
Specificity
This antibody is specific for rat CD147, which has a broad distribution; OX-47 labels T and B cell blasts, peritoneal macrophages, the endothelium of all except large vessels and kidney tubules, but does not label TDL and thymocytes.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD147 recombinant antibody
WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), RIA (Radioimmunoassay)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blotusinganti-CD147(Basigin)antibodyOX-47(AAA72471)Ratthymustissuelysate(35ugproteininRIPAbuffer)wasresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitversionofOX-47(AAA72471)at0.1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedbandsizeforCD147is42.4kDa.AAA72471successfullydetectedCD147inratthymustissuelysate.)
WB (Western Blot)
(Western Blotusinganti-CD147(Basigin)antibodyOX-47(AAA72471)Ratthymustissuelysate(35ugproteininRIPAbuffer)wasresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitversionofOX-47(AAA72471)at0.1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedbandsizeforCD147is42.4kDa.AAA72471successfullydetectedCD147inratthymustissuelysate.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocytecellswithanti-CD147(Basigin)antibodyOX-47(AAA72471) Immunofluorescenceanalysisofparaformaldehydefixedrat(Rattusnorvegicus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofOX-47(AAA72471)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembranestainingofasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72471,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocytecellswithanti-CD147(Basigin)antibodyOX-47(AAA72471) Immunofluorescenceanalysisofparaformaldehydefixedrat(Rattusnorvegicus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofOX-47(AAA72471)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembranestainingofasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72471,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusinganti-CD147(Basigin)antibodyOX-47(AAA72471) Stainingofrat(Rattusnorvegicus)leukocyteswitheitheranisotypecontrol(A)ortherabbit-chimericversionofOX-47(B,AAA72471)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusinganAlexaFluor488conjugateddonkeyanti-rabbitantibody(ab150073)andcellsanalysedonaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusinganti-CD147(Basigin)antibodyOX-47(AAA72471) Stainingofrat(Rattusnorvegicus)leukocyteswitheitheranisotypecontrol(A)ortherabbit-chimericversionofOX-47(B,AAA72471)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusinganAlexaFluor488conjugateddonkeyanti-rabbitantibody(ab150073)andcellsanalysedonaFACSCantoflow-cytometer.)
Related Product Information for CD147 recombinant antibody
Mice immunized with rat T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.
References
Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 M r detected only on CD4 positive T blasts Molecular Immunology, 1987, Vol.24(12), pp.1281-1290 N/A2828930
Note on publication:
Describes the original use of this antibody as part of FC and IP studies to identify new markers for activated T cells.
Note on publication:
Describes the original use of this antibody as part of FC and IP studies to identify new markers for activated T cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,585 Da
NCBI Official Full Name
basigin isoform 1
NCBI Official Synonym Full Names
basigin (Ok blood group)
NCBI Official Symbol
Bsg
NCBI Official Synonym Symbols
CE9; HT7; 5A11; Ox47R; EMMPRIN; basignin
NCBI Protein Information
basigin; Basigin (Ox47 antigen or CE-9) (EMMPRIN in human) (neurothelin HT7 or 5A11 in avian); Basignin (Ox47 antigen or CE-9) (EMMPRIN in human) (neurothelin HT7 or 5A11 in avian); OX-47 antigen; glycoprotein CE9
UniProt Protein Name
Basigin
UniProt Gene Name
Bsg
UniProt Entry Name
BASI_RAT
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Product Notes
The CD147 bsg (Catalog #AAA72471) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD147 [OX-47], Rabbit IgG, kappa reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD147 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), RIA (Radioimmunoassay). Researchers should empirically determine the suitability of the CD147 bsg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD147, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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