Mouse anti-Human CD158d Monoclonal Antibody | anti-CD158d antibody

Anti-Hu CD158d Purified Low Endotoxin

Cat. Num. AAA129123

• Gene Names: KIR2DL4; G9P; CD158D; KIR103; KIR-2DL4; KIR103AS; KIR-103AS
• Reactivity: Human
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay, Functional Assay, Immunocytochemistry, Western Blot
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD158d, Antibody; Anti-Hu CD158d Purified Low Endotoxin; KIR2DL4; KIR103AS; 103AS; 15.212; killer cell immunoglobulin like receptor, two Ig d; anti-CD158d antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: mAb#33
• Specificity: The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d/KIR2DL4, a 45kDa NK cell marker. Cell surface expression and Functional Application (FUNC)tion of CD158d/KIR2DL4 depends on genotype of particular individuals.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified Low Endotoxin. Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD158d antibody: IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), FA (Functional Assay), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: NK3.3 cells and KIR2DL4-Ig fusion protein
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Related Product Information for anti-CD158d antibody

CD158d/KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different Functional Application (FUNC)tions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory Functional Application (FUNC)tion, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-gamma secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.

References

*Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO. Activation of NK cells by an endocytosed receptor for soluble HLA-G. PLoS Biol. 2006 Jan;4(1):e9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16366734itool,*Yan WH, Fan LA: Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition. Cell Res. 2005 Mar;15(3):176-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/15780179itool,*LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB J. 2005 Apr;19(6):662-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/15670976itool,*Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells. J Immunol. 2001 Aug 15;167(4):1877-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/11489965itool,*Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and Functional Application (FUNC)tion in NK cells. J Immunol. 2003 Aug 15;171(4):1768-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/12902476itool

NCBI and Uniprot Product Information

• NCBI GI #: 325511396
• NCBI GeneID: 3805
• UniProt Accession #: Q99706; O14621; O14622; O14623; O14624; O43534; P78400; P78401; Q8N738; Q99559; Q99560; A8W795
• Molecular Weight: 24,917 Da
• NCBI Official Full Name: Killer cell immunoglobulin-like receptor 2DL4
• NCBI Official Synonym Full Names: killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4
• NCBI Official Symbol: KIR2DL4
• NCBI Official Synonym Symbols: G9P; CD158D; KIR103; KIR-2DL4; KIR103AS; KIR-103AS
• NCBI Protein Information: killer cell immunoglobulin-like receptor 2DL4
• UniProt Protein Name: Killer cell immunoglobulin-like receptor 2DL4
• UniProt Gene Name: KIR2DL4
• UniProt Synonym Gene Names: CD158D; KIR103AS; KIR-103AS