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WB (Western Blot)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.)
Mouse anti-Human CD163 Monoclonal Antibody | anti-CD163 antibody
Mouse Monoclonal [clone 3B4] (IgG2a) to Human CD163
Gene Names
CD163; M130; MM130
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Protein A/G Purified
Synonyms
CD163, Antibody; Mouse Monoclonal [clone 3B4] (IgG2a) to Human CD163; Anti-CD163 Antibody (clone 3B4) IHC-plus; CD163; CD163 antigen; Macrophage-associated antigen; M130; CD163 molecule; Hemoglobin scavenger receptor; MM130; Human CD163; anti-CD163 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3B4
Specificity
Human CD163
Purity/Purification
Protein A/G Purified
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
1156
Applicable Applications for anti-CD163 antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Full length human recombinant protein of human CD163(NP_004235) produced in HEK293T cell.
Immunogen Type
Recombinant protein
Immunogen
CD163 antibody was raised against full length human recombinant protein of human CD163(NP_004235) produced in HEK293T cell.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
WB (Western Blot)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.)
WB (Western Blot)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.)
WB (Western Blot)
(Western blot of extracts (35 ug) from 9 different cell lines by using anti-CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
WB (Western Blot)
(Western blot of extracts (35 ug) from 9 different cell lines by using anti-CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
IF (Immunofluorescence)
(Anti-CD163 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163.)
IF (Immunofluorescence)
(Anti-CD163 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163.)
IHC (Immunohistochemistry)
(Anti-CD163 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-CD163 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
124,958 Da
NCBI Official Full Name
scavenger receptor cysteine-rich type 1 protein M130 isoform a
NCBI Official Synonym Full Names
CD163 molecule
NCBI Official Symbol
CD163
NCBI Official Synonym Symbols
M130; MM130
NCBI Protein Information
scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
UniProt Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
UniProt Gene Name
CD163
UniProt Synonym Gene Names
M130; sCD163
UniProt Entry Name
C163A_HUMAN
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Product Notes
The CD163 cd163 (Catalog #AAA52558) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 3B4] (IgG2a) to Human CD163 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD163 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD163 cd163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD163, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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