FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD18 antibody 1B4. Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 1B4 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Mouse CD18 Monoclonal Antibody | anti-CD18 antibody
Anti-CD18 [1B4], Mouse IgG2a, Kappa
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Human, Rhesus Monkey
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, immunoassay, Radioimmunoassay, Immunoprecipitation
Purity
Protein A affinity purified
Synonyms
CD18, Antibody; Anti-CD18 [1B4], Mouse IgG2a, Kappa; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; ITGB2; MFI7; IB4; m1B4; mIB4; anti-CD18 antibody
Host
Mouse
Reactivity
Human, Rhesus Monkey
Clonality
Monoclonal
Isotype
Mouse IgG2a, Kappa
Clone Number
1B4
Specificity
Specific to the beta chain of the leukocyte integrin beta-2 family. This is found in LFA-1, Mac-1 and p150.95 among others. Binds an epitope distinct from the C3bi binding site. Has been shown to recognise both the human and Rhesus monkey antigen.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD18 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, RIA (Radioimmunoassay), IP (Immunoprecipitation)
Antibody Type
Recombinant Antibody
Immunogen
Blood was obtained from 4 helathy donors and the mononuclear cells harvested and grown. A BALB/c mouse was injected with 10^6 monocytes three times at monthly intervals, and four times with 5x10^6 monocytes oon each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD18 antibody 1B4. Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 1B4 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD18 antibody 1B4. Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 1B4 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of human peripheral blood monocytes using anti-CD18 antibody 1B4 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of 1B4 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of human peripheral blood monocytes using anti-CD18 antibody 1B4 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of 1B4 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD18 antibody
Describes the generation of this IB4 and others, analysis of their binding by competitive binding experiments to identify different/common epitopes, immunoprecipitation and ELISAs to confirm a common antigen.
Recombinant monoclonal antibody to CD18. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1B4.
Recombinant monoclonal antibody to CD18. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1B4.
Product Categories/Family for anti-CD18 antibody
References
Wright et al; Proc Natl Acad Sci USA. 1983 Sep; 80(18): 5699-5703. OriginPubPMID: 8095956
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,782 Da
NCBI Official Full Name
integrin beta-2
NCBI Official Synonym Full Names
integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
NCBI Official Symbol
ITGB2
NCBI Official Synonym Symbols
LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
NCBI Protein Information
integrin beta-2; integrin beta chain, beta 2; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; cell surface adhesion glycoproteins
UniProt Protein Name
Integrin beta-2
UniProt Gene Name
ITGB2
UniProt Synonym Gene Names
CD18; MFI7
UniProt Entry Name
ITB2_HUMAN
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Product Notes
The CD18 itgb2 (Catalog #AAA72058) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD18 [1B4], Mouse IgG2a, Kappa reacts with Human, Rhesus Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD18 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, RIA (Radioimmunoassay), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the CD18 itgb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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