FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD18a)
Mouse anti-Pig CD18a Monoclonal Antibody | anti-CD18a antibody
MOUSE ANTI PIG CD18a
Gene Names
ITGB2; CD18
Reactivity
Pig
Applications
Immunoprecipitation, Functional Assay, Flow Cytometry, Functional Assay
Synonyms
CD18a, Antibody; MOUSE ANTI PIG CD18a; anti-CD18a antibody
Host
Mouse
Reactivity
Pig
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PNK-I
Specificity
CD18a
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
769
Applicable Applications for anti-CD18a antibody
IP (Immunoprecipitation), FA (Functional Assay), FCM/FACS (Flow Cytometry)
Reactivity Notes
Camel
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD18a)
FCM/FACS (Flow Cytometry)
(Staining of porcine peripheral blood granulocytes with Mouse anti Porcine CD18a)
FCM/FACS (Flow Cytometry)
(Staining of peripheral blood lymphocytes with Mouse anti Pig CD18a followed by Goat anti Mouse IgG:FITC)
FCM/FACS (Flow Cytometry)
(Staining of peripheral blood lymphocytes with Mouse anti Pig CD18a followed by Goat anti Mouse IgG:FITC)
Related Product Information for anti-CD18a antibody
Mouse anti Pig CD18a, clone PNK-I recognizes porcine CD18a. PNK-I was clustered as CD18a at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998). Clone PNK-I immunoprecipitates proteins of ~166 kDa, ~155 kDa and ~95 kDa under non-reducing conditions, specifically recognizing the 95 kDa protein, consistent with the integrin beta2 chain (CD18). PNK-I inhibits porcine NK cell activity independent of any effect on antibody dependent cellular cytotoxicity (Dato and Kim 1990). CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules. CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor (Chen et al. 2011).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
84,790 Da
NCBI Official Full Name
Integrin beta-2
NCBI Official Symbol
ITGB2
NCBI Official Synonym Symbols
CD18
NCBI Protein Information
integrin beta-2
UniProt Protein Name
Integrin beta-2
UniProt Gene Name
ITGB2
UniProt Synonym Gene Names
CD18
UniProt Entry Name
ITB2_PIG
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Product Notes
The CD18a itgb2 (Catalog #AAA50258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI PIG CD18a reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CD18a can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FA (Functional Assay), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD18a itgb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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