Rat CD19 Monoclonal Antibody | anti-CD19 antibody

RAT ANTI MOUSE CD19

Cat. Num. AAA11932

• Gene Names: Cd19; AW495831
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD19, Antibody; RAT ANTI MOUSE CD19; anti-CD19 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 6D5
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 547

• Applicable Applications for anti-CD19 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: Human K562 cell line transfected with murine CD19; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 , green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using DAPI. Low power)

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD19:RPE-Alexa Fluor 750)

Application Data

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 , green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using DAPI. High power)

Application Data

(Immunoperoxidase staining of a murine lymph node with Rat anti Mouse CD19 antibody, clone 6D5 forllowerd by horseradish peroxidase conjugated Goat anti Rat IgG . Low power)

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD19)

Application Data

(Immunoperoxidase staining of a murine lymph node with Rat anti Mouse CD19 antibody, clone 6D5 forllowerd by horseradish peroxidase conjugated Goat anti Rat IgG . High power)

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD19:APC)

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD19:FITC)

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD19:RPE-Alexa Fluor 647)

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD19:RPE)

NCBI and Uniprot Product Information

• NCBI GI #: 146149173
• NCBI GeneID: 12478
• NCBI Accession #: NP_033974.2
• NCBI GenBank Nucleotide #: NM_009844.2
• Molecular Weight: 60,149 Da
• NCBI Official Full Name: B-lymphocyte antigen CD19
• NCBI Official Synonym Full Names: CD19 antigen
• NCBI Official Symbol: Cd19
• NCBI Official Synonym Symbols: AW495831
• NCBI Protein Information: B-lymphocyte antigen CD19; differentiation antigen CD19
• UniProt Protein Name: B-lymphocyte antigen CD19
• UniProt Gene Name: Cd19
• UniProt Entry Name: CD19_MOUSE