Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD195:FITC)
Rat anti-Human CD195 Monoclonal Antibody | anti-CD195 antibody
RAT ANTI HUMAN CD195
Gene Names
CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Reactivity
Human
Applications
Flow Cytometry
Synonyms
CD195, Antibody; RAT ANTI HUMAN CD195; anti-CD195 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
HEK/1/85a
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
352
Applicable Applications for anti-CD195 antibody
Flow cytometry (FC)
Application Notes
FC: 1/50-1/100
Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul fresh whole blood.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul fresh whole blood.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
CHO cells transfected with human CCR5.
Fusion Partners
Spleen cells from immunised rats were fused with cell of the rat Y3 myeloma cell line.
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD195:FITC)
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD195:FITC)
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD195: Azide Free)
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD195: Azide Free)
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD195:RPE)
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD195:RPE)
Related Product Information for anti-CD195 antibody
Rat anti Human CD195 antibody, clone HEK/1/85a recognizes the human CD195 cell surface antigen, a 45kDa glycoprotein also known as CCR5. CD195 acts as a receptor for a number of chemokines including RANTES and eotaxin and also serves as a co-receptor for the entry of HIV into cells. CD195 is expressed by a subset of T lymphocytes and by monocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-C chemokine receptor type 5
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 5 (gene/pseudogene)
NCBI Official Symbol
CCR5
NCBI Official Synonym Symbols
CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
NCBI Protein Information
C-C chemokine receptor type 5; C-C motif chemokine receptor 5 A159A; HIV-1 fusion coreceptor; chemokine receptor CCR5; chemr13
UniProt Protein Name
C-C chemokine receptor type 5
UniProt Gene Name
CCR5
UniProt Synonym Gene Names
CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5
UniProt Entry Name
CCR5_HUMAN
Similar Products
Product Notes
The CD195 ccr5 (Catalog #AAA11952) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RAT ANTI HUMAN CD195 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD195 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC). FC: 1/50-1/100 Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul fresh whole blood. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD195 ccr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD195, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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