Mouse anti-Human CD20/MS4A1 Monoclonal Antibody | anti-MS4A1 antibody

CD20/MS4A1 (B-Cell Marker)

Cat. Num. AAA23946

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD20/MS4A1; Monoclonal Antibody; CD20/MS4A1 (B-Cell Marker); APY; ATOPY; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; Leukocyte surface antigen Leu-16; Ly44; MS4A1; MS4A2; anti-MS4A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: L26
• Specificity: Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 297

• Applicable Applications for anti-MS4A1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Daudi, Raji and U266 cell lines. Human lymphocytes. Human lymph node or tonsil.
• Immunogen: Human tonsil B cells
• Cellular Localization: Predominantly Cell Surface with some cytoplasmic
• Hu-chromosome Location: 11q12.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

WB (Western Blot)

(Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).)

IF (Immunofluorescence)

(Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)

SDS-PAGE

(SDS-PAGE Analysis Purified CD20 Mouse Monoclonal Antibody (L26). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (L26).)

IF (Immunofluorescence)

(Cytometric Analysis of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (L26).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (L26).)

References

Schlossman, S., et al., eds. 1995. Leucocyte Typing V. New York: Oxford University Press.

NCBI and Uniprot Product Information

• NCBI GI #: 115968
• NCBI GeneID: 931
• UniProt Accession #: P11836
• Molecular Weight: 33-37kDa
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane spanning 4-domains A1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20
• UniProt Protein Name: B-lymphocyte antigen CD20
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN