Mouse anti-Human CD20/MS4A1 Monoclonal Antibody | anti-MS4A1 antibody

CD20/MS4A1 (B-Cell Marker)

Cat. Num. AAA23945

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD20/MS4A1, Antibody; CD20/MS4A1 (B-Cell Marker); APY; ATOPY; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1); anti-MS4A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: MS4A1/3409
• Specificity: Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 297

• Applicable Applications for anti-MS4A1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA); FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml; WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Jurkat, Daudi, Raji or U266 cells. Lymph node and Tonsil.
• Immunogen: A recombinant fragment (around aa 213-297) of human MS4A1 protein (exact sequence is proprietary)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 11q12.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD20 Mouse Recombinant Monoclonal Antibody (MS4A1/3409). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

WB (Western Blot)

(Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (MS4A1/3409).)

IF (Immunofluorescence)

(Immunofluorescence staining of Raji cells usin CD20 Mouse Monoclonal Antibody (MS4A1/3409) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of Raji cells using CD20 Mouse Monoclonal Antibody (MS4A1/3409) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

SDS-PAGE

(SDS-PAGE Analysis Purified CD20 Mouse Monoclonal Antibody (MS4A1/3409). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (MS4A1/3409).)

References

Tedder, T.F., et al. 1994. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol. Today 15: 450-454

NCBI and Uniprot Product Information

• NCBI GI #: 115968
• NCBI GeneID: 931
• UniProt Accession #: P11836
• Molecular Weight: 33-37kDa
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane spanning 4-domains A1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20
• UniProt Protein Name: B-lymphocyte antigen CD20
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN