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Application Data
(Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD20.)
B-lymphocyte antigen CD20 (MS4A1) Recombinant Protein | MS4A1 recombinant protein
Recombinant Human B-lymphocyte antigen CD20 (MS4A1)-VLPs (Active)
Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Purity
The purity information is not available for VLPs proteins.
Synonyms
B-lymphocyte antigen CD20 (MS4A1); N/A; Recombinant Human B-lymphocyte antigen CD20 (MS4A1)-VLPs (Active); B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20; MS4A1 recombinant protein
Host
Mammalian cell
Purity/Purification
The purity information is not available for VLPs proteins.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Sequence Positions
1-297aa; Full Length
Sequence
MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP
Species
Homo sapiens (Human)
Tag
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Research Area
CancerLess than 1.0 EU/ug as determined by LAL method.
Transmembrane Domain
4TM
Biological_Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD20 at 2 ug/mL can bind Anti-CD20 recombinant antibody, the EC50 is 3.243-7.085 ng/mL.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Application Data
(Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD20.)
Application Data
(Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD20.)
Application Data
(Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD20.)
Application Data
(Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) CD20.)
Application Data
(The presence of VLP-like structures was confirmed by TEM)
Application Data
(The presence of VLP-like structures was confirmed by TEM)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized human CD20 at 2 ug/ml can bind Anti-CD20 recombinant antibody , the EC50 is 3.243-7.085 ng/mL.)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized human CD20 at 2 ug/ml can bind Anti-CD20 recombinant antibody , the EC50 is 3.243-7.085 ng/mL.)
WB (Western Blot)
(Western Blotis detected by Mouse anti-6*His monoclonal antibody.)
WB (Western Blot)
(Western Blotis detected by Mouse anti-6*His monoclonal antibody.)
Product Categories/Family for MS4A1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
297
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane-spanning 4-domains, subfamily A, member 1
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
NCBI Protein Information
B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_HUMAN
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Product Notes
The MS4A1 ms4a1 (Catalog #AAA279270) is a Recombinant Protein produced from Mammalian cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-297aa; Full Length. The amino acid sequence is listed below: MTTPRNSVNG TFPAEPMKGP IAMQSGPKPL FRRMSSLVGP TQSFFMRESK TLGAVQIMNG LFHIALGGLL MIPAGIYAPI CVTVWYPLWG GIMYIISGSL LAATEKNSRK CLVKGKMIMN SLSLFAAISG MILSIMDILN IKISHFLKME SLNFIRAHTP YINIYNCEPA NPSEKNSPST QYCYSIQSLF LGILSVMLIF AFFQELVIAG IVENEWKRTC SRPKSNIVLL SAEEKKEQTI EIKEEVVGLT ETSSQPKNEE DIEIIPIQEE EEEETETNFP EPPQDQESSP IENDSSP. It is sometimes possible for the material contained within the vial of "B-lymphocyte antigen CD20 (MS4A1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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