Rabbit anti-Mouse, Human Mannose Receptor / CD206 Polyclonal Antibody | anti-CD206 antibody

Anti-Mannose Receptor / CD206 Antibody (aa358-388) IHC-plus

Cat. Num. AAA52636

• Gene Names: MRC1; MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
• Reactivity: Mouse, Human
• Applications: Western Blot, Immunohistochemistry
• Purity: P{rotein A purified
• Synonyms: Mannose Receptor / CD206, Antibody; Anti-Mannose Receptor / CD206 Antibody (aa358-388) IHC-plus; Rabbit Polyclonal to Human Mannose Receptor / CD206; Human Mannose Receptor / CD206; MRC1; BA541I19.1; CLEC13D; CD206 antigen; Macrophage mannose receptor 1; Mannose Receptor; Macrophage mannose receptor; MMR; Mannose receptor; C type 1; MRC1L1; CLEC13DL; anti-CD206 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Specificity: This MRC1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-388 amino acids from the N-terminal region of human MRC1L1.
• Purity/Purification: P{rotein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 1456

• Applicable Applications for anti-CD206 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen: Mannose Receptor / CD206 antibody was raised against Human
• Antigen Modification: aa358-388
• Target: Mannose Receptor / CD206
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

WB (Western Blot)

(MRC1L1 Antibody western blot of mouse lung tissue lysates (35 ug/lane). The MRC1L1 antibody detected the MRC1L1 protein (arrow).)

IHC (Immunohistochemistry)

(Anti-Mannose Receptor / CD206 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Related Product Information for anti-CD206 antibody

MRC1 Antibody, BA541I19.1 Antibody, CLEC13D Antibody, CD206 Antibody, CD206 antigen Antibody, Macrophage mannose receptor 1 Antibody, Mannose Receptor Antibody, Macrophage mannose receptor Antibody, MMR Antibody, Mannose receptor, C type 1 Antibody, MRC1L1 Antibody, CLEC13DL Antibody Description: The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages.

NCBI and Uniprot Product Information

• NCBI GI #: 4505245
• NCBI GeneID: 4360
• NCBI Accession #: NP_002429.1
• NCBI GenBank Nucleotide #: NM_002438.3
• UniProt Accession #: P22897; Q5VSJ2; Q5VSK2; A5PKW3
• NCBI Official Full Name: macrophage mannose receptor 1
• NCBI Official Synonym Full Names: mannose receptor, C type 1
• NCBI Official Symbol: MRC1
• NCBI Official Synonym Symbols: MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
• NCBI Protein Information: macrophage mannose receptor 1
• UniProt Protein Name: Macrophage mannose receptor 1
• UniProt Gene Name: MRC1
• UniProt Synonym Gene Names: CLEC13D; CLEC13DL; MRC1L1; MMR; hMR
• UniProt Entry Name: MRC1_HUMAN