Mouse CD22/BL-CAM Monoclonal Antibody | anti-CD22 antibody

CD22/BL-CAM (B-Cell Marker)

Cat. Num. AAA23895

• Gene Names: CD22; SIGLEC2; SIGLEC-2
• Reactivity: Human. Others not known.
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: CD22/BL-CAM, Antibody; CD22/BL-CAM (B-Cell Marker); B-lymphocyte cell adhesion molecule (BL-CAM); B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2); SIGLEC2; T-cell surface antigen Leu-14; anti-CD22 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: BLCAM/1795
• Specificity: Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
• Form/Format: 1.0mg/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
• Sequence Length: 759

• Applicable Applications for anti-CD22 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined
• Immunogen: Recombinant fragment of human BL-CAM/CD22 protein (around aa 52-178)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 19q13.1
• Positive Control: Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human Tonsil, Lymph Node or Spleen.
• Preparation and Storage: Antibody without azide - store at -20°C to -80°C.; Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)

ELISA

(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) Z-and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descendingorder of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of paraformaldehyde-fixed MOLT4 cells. CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

SDS-PAGE

(SDS-PAGE Analysis Purified CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795). Confirmation of Purity and Integrity of Antibody)

WB (Western Blot)

(Western Blot Analysis of Raji cell lysate using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).)

WB (Western Blot)

(Western Blot Analysis of Raji and Ramos Cell Lysates using CD22 Mouse Monoclonal Antibody (BLCAM/1795).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD22 Mouse Monoclonal Antibody (BLCAM/1795).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Spleen stained with CD22 Mouse Monoclonal Antibody (BLCAM/1795).)

References

Sato S et al. CD22 negatively and positively regulates signal transduction through the B lymphocyte antigen receptor. Semin Immunol 1998, 10:287-297.

NCBI and Uniprot Product Information

• NCBI GI #: 297374825
• NCBI GeneID: 933
• NCBI Accession #: NP_001172028.1
• NCBI GenBank Nucleotide #: NM_001185099.1
• UniProt Accession #: P20273; O95699; O95701; O95702; O95703; Q01665; Q32M46; Q92872; Q92873; Q9UQA6; F5GYU4; F5H7U3
• Molecular Weight: 130-140kDa
• NCBI Official Full Name: B-cell receptor CD22 isoform 2
• NCBI Official Synonym Full Names: CD22 molecule
• NCBI Official Symbol: CD22
• NCBI Official Synonym Symbols: SIGLEC2; SIGLEC-2
• NCBI Protein Information: B-cell receptor CD22
• UniProt Protein Name: B-cell receptor CD22
• UniProt Gene Name: CD22
• UniProt Synonym Gene Names: SIGLEC2; BL-CAM; Siglec-2