IF (Immunofluorescence)
(Immunofluorescence staining of fixed mouse splenocytes with anti- CD226 antibody 10E5 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of 10E5 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Rat anti-Mouse CD226 Monoclonal Antibody | anti-CD226 antibody
Anti-CD226 [10E5]
Gene Names
Cd226; Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD226, Antibody; Anti-CD226 [10E5]; DNAM-1; PTA1; PTA-1; platelet and T cell activation antigen 1; TLISA1; anti-CD226 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b, kappa
Clone Number
1000000
Specificity
This antibody is specific for murine CD226.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD226 antibody
FCM/FACS (Flow Cytometry)
PMID
16034094
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of fixed mouse splenocytes with anti- CD226 antibody 10E5 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of 10E5 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed mouse splenocytes with anti- CD226 antibody 10E5 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of 10E5 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-CD266 antibody 10E5 Mouse spleen tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 10E5 at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CD266 antibody 10E5 Mouse spleen tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 10E5 at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Related Product Information for anti-CD226 antibody
Recombinant monoclonal antibody to CD226. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 10E5.
This antibody was raised by immunising Lewis and Lou/M rats with the murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice, before screening for reactivity with Th1 clones and a lack of reactivity to Th2/Th0 cell lines.
This antibody was raised by immunising Lewis and Lou/M rats with the murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice, before screening for reactivity with Th1 clones and a lack of reactivity to Th2/Th0 cell lines.
References
Dardalhon et al. CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. J Immunol. 2005 Aug 1;175(3):1558-65.
Note on publication: Describes the original generation of this antibody, and its characterisation.
Note on publication: Describes the original generation of this antibody, and its characterisation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,063 Da
NCBI Official Full Name
CD226 antigen isoform a
NCBI Official Synonym Full Names
CD226 antigen
NCBI Official Symbol
Cd226
NCBI Official Synonym Symbols
Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
NCBI Protein Information
CD226 antigen; platelet and T cell activation antigen 1; platelet and T-cell activation antigen 1
UniProt Protein Name
CD226 antigen
UniProt Gene Name
Cd226
UniProt Synonym Gene Names
Pta1
UniProt Entry Name
CD226_MOUSE
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Product Notes
The CD226 cd226 (Catalog #AAA72126) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD226 [10E5] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD226 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD226 cd226 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD226, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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