Application Data
(Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 as a capture reagent and biotinylated Mouse anti Human CD227, clone 6A4 as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma and serum samples diluted 1:5 are indicated in green and orange respectively)
Mouse CD227 Monoclonal Antibody | anti-CD227 antibody
MOUSE ANTI HUMAN CD227:FITC
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Applications
Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
CD227, Antibody; MOUSE ANTI HUMAN CD227:FITC; anti-CD227 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG3
Clone Number
C595 (NCRC48)
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
264
Applicable Applications for anti-CD227 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 as a capture reagent and biotinylated Mouse anti Human CD227, clone 6A4 as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma and serum samples diluted 1:5 are indicated in green and orange respectively)
Application Data
(Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 as a capture reagent and biotinylated Mouse anti Human CD227, clone 6A4 as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma and serum samples diluted 1:5 are indicated in green and orange respectively)
Application Data
(Immunofluorescence staining of MCF-7 cells with Mouse anti Human CD227:FITC (green), nuclei are counterstained with DAPI (blue))
Application Data
(Immunofluorescence staining of MCF-7 cells with Mouse anti Human CD227:FITC (green), nuclei are counterstained with DAPI (blue))
Application Data
(Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 as a capture reagent and biotinylated Mouse anti Human CD227, clone VU-4H5 as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively)
Application Data
(Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 as a capture reagent and biotinylated Mouse anti Human CD227, clone VU-4H5 as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively)
Related Product Information for anti-CD227 antibody
Mouse anti Human CD227 antibody, clone C595 (NCRC48) recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases. Mouse anti Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,297 Da
NCBI Official Full Name
mucin-1 isoform 2
NCBI Official Synonym Full Names
mucin 1, cell surface associated
NCBI Official Symbol
MUC1
NCBI Official Synonym Symbols
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
NCBI Protein Information
mucin-1; DF3 antigen; H23 antigen; Medullary cystic kidney disease, autosomal dominant; breast carcinoma-associated antigen DF3; cancer antigen 15-3; carcinoma-associated mucin; episialin; krebs von den Lungen-6; mucin 1, transmembrane; peanut-reactive urinary mucin; polymorphic epithelial mucin; tumor associated epithelial mucin; tumor-associated epithelial membrane antigen
UniProt Protein Name
Mucin-1
UniProt Gene Name
MUC1
UniProt Synonym Gene Names
PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta
UniProt Entry Name
MUC1_HUMAN
Similar Products
Product Notes
The CD227 muc1 (Catalog #AAA49064) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD227 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD227 muc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD227, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.