WB (Western Blot)
(Western Blot using Anti-CD25 antibody 2E4 . Mouse lymph node tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 2E4 at 0.5ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Mouse anti-Mouse CD25 Monoclonal Antibody | anti-CD25 antibody
Anti-CD25 [2E4]
Gene Names
Il2ra; CD25; Il2r; Ly-43
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD25, Antibody; Anti-CD25 [2E4]; IL-2-RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2-RA; p55; anti-CD25 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
20000
Specificity
2E4 recognizes mouse CD25. This epitope is distinct from the IL-2 binding site and does not block binding of IL-2 to CD25. This antibody binds the same or overlapping epitope as 7D4 (Ab02216) but different than Ab01107. Murine CD25 is a 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD25 antibody
FCM/FACS (Flow Cytometry)
PMID
6206144
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blot using Anti-CD25 antibody 2E4 . Mouse lymph node tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 2E4 at 0.5ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using Anti-CD25 antibody 2E4 . Mouse lymph node tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 2E4 at 0.5ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD25 antibody 2E4 . Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 2E4 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD25 antibody 2E4 . Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 2E4 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-CD25 antibody
Recombinant monoclonal antibody to CD25. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2E4.
The original version of this antibody was raised to Recognise mouse CD25.
The original version of this antibody was raised to Recognise mouse CD25.
Product Categories/Family for anti-CD25 antibody
References
Ortega et al. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells J Immunol. 1984 Oct;133(4):1970-5.
Note on publication: Describes the characterization of the antibody 2E4.
Note on publication: Describes the characterization of the antibody 2E4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,723 Da
NCBI Official Full Name
Interleukin-2 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha chain
NCBI Official Symbol
Il2ra
NCBI Official Synonym Symbols
CD25; Il2r; Ly-43
NCBI Protein Information
interleukin-2 receptor subunit alpha; IL2-RA; IL-2-RA; p55 chain; IL-2R alpha chain; IL-2R subunit alpha; IL-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Gene Name
Il2ra
UniProt Synonym Gene Names
Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_MOUSE
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Product Notes
The CD25 il2ra (Catalog #AAA72226) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD25 [2E4] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD25 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD25 il2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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