Mouse anti-Human CD252 Monoclonal Antibody | anti-CD252 antibody

anti-human CD252(OX40L)-R-PE

Cat. Num. AAA78153

• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Synonyms: CD252, Antibody; anti-human CD252(OX40L)-R-PE; Monoclonal anti-human CD252 (CD134 L, OX40 L)/R-PE; anti-CD252 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: ANC10G1
• Form/Format: 0 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

• Applicable Applications for anti-CD252 antibody: Blocking, ELISA, FCM/FACS (Flow Cytometry)
• Performance: Cultured human umbilical cord vein endothelial cells were harvested by trypsinization, after which 5 x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD252/R-PE at a dilution factor of 1:50 (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 63% of the cells stained positive with a mean shift of 0.83 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD252 antibody.
• Conjugation: PE
• Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A₅₆₅/A₂₈₀ ratio of 3.02.
• Immunogen: Human HUVEC and Recombinant soluble human CD252
• Preparation and Storage: Store at 2 - 5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after receiving date when stored as recommended.

Application Data

Related Product Information for anti-CD252 antibody

Information: Human CD252 (CD134L, OX-40L) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD252 binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of inflammation and rejection (5,6,7,8). Antibody from clone ANC10G1 binds to CD252 on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.

References

1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2) L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3) A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826. 4) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 5) V. Malmstrom, et al, (2001) J Immunol 166: 6972. 6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115. 7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955. 8) L. Tian, et al, (2002) Transplantation 74(1): 133-138.