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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Daudi cells with CD27 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Rat CD27 Monoclonal Antibody | anti-CD27 antibody
CD27 Antibody
Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Reactivity
Human, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
CD27, Antibody; CD27 Antibody; CD 27 antibody; CD27 antibody; CD27 antigen antibody; CD27 molecule antibody; CD27_HUMAN antibody; CD27L receptor antibody; LPFS2 antibody; MGC20393 antibody; OTTHUMP00000238557 antibody; S152 antibody; T cell activation antigen CD27 antibody; T cell antivation antigen S152 antibody; T-cell activation antigen CD27 antibody; T14 antibody; TNFRSF 7 antibody; TNFRSF7 antibody; TNFSF7 antibody; Tp 55 antibody; Tp55 antibody; Tumor necrosis factor receptor superfamily member 7 antibody; anti-CD27 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Clone Number
JB40-98
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CD27 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Intracellular domain corresponding to Human CD27.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Daudi cells with CD27 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Daudi cells with CD27 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining CD27 in K562 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CD27 in K562 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD27 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD27 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD27 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD27 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of CD27 on Raji cell using anti-CD27 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of CD27 on Raji cell using anti-CD27 antibody at 1/500 dilution.)
Related Product Information for anti-CD27 antibody
The tumor necrosis factor (TNF) receptor family is composed of several type I integral membrane glycoproteins that exhibit homology in their cysteine-rich extracellular domains. Members of this family include FAS, OX40, CD27 and CD30. Ligands for these receptors are often type II transmembrane glycoproteins, as is the case for CD27 and CD30. CD27 is a homodimeric lymphocyte-specific surface antigen present on T and B lymphocytes. Activation of the CD3 complex via the T cell receptor for antigen leads to an increase in CD27 expression. Together, CD27 and its ligand, CD27L, generate co-stimulatory signals required for complete T cell activation. CD30 is a surface marker for neoplastic cells of the Hodgkin's lymphoma and related hematologic malignancies. CD30L has been shown to enhance the proliferation of the Hodgkin's cell line HDLM-2, but exerts antiproliferative effects on large cell anaplastic lymphoma cell lines.
Product Categories/Family for anti-CD27 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,137 Da
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
CD27
NCBI Official Synonym Symbols
T14; S152; Tp55; TNFRSF7; S152. LPFS2
NCBI Protein Information
CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
UniProt Protein Name
CD27 antigen
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
UniProt Entry Name
CD27_HUMAN
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Product Notes
The CD27 cd27 (Catalog #AAA312639) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD27 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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