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WB (Western Blot)
(Detection of human CD27 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Ramos, HEK293T, Raji, Jurkat, K-562, U2OS, Daudi, MCF-7, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)
CD27 recombinant antibody
Rabbit anti-CD27 Recombinant Monoclonal Antibody [BLR083G]
Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CD27, Recombinant Antibody; Rabbit anti-CD27 Recombinant Monoclonal Antibody [BLR083G]; TNFRSF7; CD antigen CD27; tumor necrosis factor receptor superfamily, member 7; Tp55; T-cell activation antigen CD27; T14; CD27 antigen; S152. LPFS2; Tumor necrosis factor receptor superfamily member 7; S152; CD27L receptor; T cell activation antigen S152; CD27 molecule; CD27 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR083G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for CD27 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 21-187 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD27 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Ramos, HEK293T, Raji, Jurkat, K-562, U2OS, Daudi, MCF-7, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)
WB (Western Blot)
(Detection of human CD27 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Ramos, HEK293T, Raji, Jurkat, K-562, U2OS, Daudi, MCF-7, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)
IP (Immunoprecipitation)
(Detection of human CD27 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1) used for IP at 20 ul/mg lysate. CD27 was also immunoprecipitated by rabbit anti-CD27 antibody BL16148. For blotting immunoprecipitated CD27, AAA213567 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CD27 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1) used for IP at 20 ul/mg lysate. CD27 was also immunoprecipitated by rabbit anti-CD27 antibody BL16148. For blotting immunoprecipitated CD27, AAA213567 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry)
(Detection of human CD27 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human CD27 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD27 in FFPE Ramos cells by ICC. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD27 in FFPE Ramos cells by ICC. Antibody: Rabbit anti-CD27 recombinant monoclonal antibody (AAA213567 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human CD27 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-CD27 recombinant monoclonal (AAA213567) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human CD27 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-CD27 recombinant monoclonal (AAA213567) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for CD27 recombinant antibody
CD27 is the receptor for CD70/CD27L. It may play a role in survival of activated T-cells.It may also play a role in apoptosis through association with SIVA1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P26842].
Product Categories/Family for CD27 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,137 Da
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
CD27
NCBI Official Synonym Symbols
T14; S152; Tp55; TNFRSF7; S152. LPFS2
NCBI Protein Information
CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
UniProt Protein Name
CD27 antigen
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
UniProt Entry Name
CD27_HUMAN
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Product Notes
The CD27 cd27 (Catalog #AAA213567) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD27 Recombinant Monoclonal Antibody [BLR083G] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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