IF (Immunofluorescence)
(Immunocytochemistry analysis of CD273 staining in PD-L2-transfected 293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.)
Mouse anti-Human CD273 Monoclonal Antibody | anti-CD273 antibody
Anti-CD273 Antibody
Gene Names
PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
This antibody is purified through a protein G column.
Synonyms
CD273, Antibody; Anti-CD273 Antibody; anti-CD273 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Specificity
Recognizes endogenous levels of CD273 protein.
Purity/Purification
This antibody is purified through a protein G column.
Form/Format
Mouse IgG1 kappa. Liquid in PBS, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-CD273 antibody
IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD273. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunocytochemistry analysis of CD273 staining in PD-L2-transfected 293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.)
IF (Immunofluorescence)
(Immunocytochemistry analysis of CD273 staining in PD-L2-transfected 293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.)
WB (Western Blot)
(Western blot analysis of CD273 expression in Nontransfected 293T (A), Transfected PDL2transfected 293T (B) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 40 kD))
WB (Western Blot)
(Western blot analysis of CD273 expression in Nontransfected 293T (A), Transfected PDL2transfected 293T (B) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 40 kD))
Related Product Information for anti-CD273 antibody
Mouse monoclonal antibody to CD273
Product Categories/Family for anti-CD273 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,745 Da
NCBI Official Full Name
programmed cell death 1 ligand 2
NCBI Official Synonym Full Names
programmed cell death 1 ligand 2
NCBI Official Symbol
PDCD1LG2
NCBI Official Synonym Symbols
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
NCBI Protein Information
programmed cell death 1 ligand 2
UniProt Protein Name
Programmed cell death 1 ligand 2
UniProt Gene Name
PDCD1LG2
UniProt Synonym Gene Names
B7DC; CD273; PDCD1L2; PDL2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC
UniProt Entry Name
PD1L2_HUMAN
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Product Notes
The CD273 pdcd1lg2 (Catalog #AAA266103) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD273 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD273 can be used in a range of immunoassay formats including, but not limited to, IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CD273 pdcd1lg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD273, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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