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Application Data
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Mouse CD284 Monoclonal Antibody | anti-CD284 antibody

MOUSE ANTI HUMAN CD284:Low Endotoxin

Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
Flow Cytometry, Functional Assay, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
CD284, Antibody; MOUSE ANTI HUMAN CD284:Low Endotoxin; anti-CD284 antibody
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
HTA125
Form/Format
Low Endotoxin
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
639
Applicable Applications for anti-CD284 antibody
Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Ba/F3 cell line expressing TLR4 (CD284).
Fusion Partners
Target Species
Human
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD284: Low Endotoxin)

Application Data

(Staining of U937 cells with Mouse anti Human CD284:RPE)

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD284)

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD284: FITC)

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor 647)

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor 488)

Related Product Information for anti-CD284 antibody
Mouse anti Human CD284 antibody, clone HTA125 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of MBS211211 for this purpose.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,301 Da
NCBI Official Full Name
toll-like receptor 4 isoform C
NCBI Official Synonym Full Names
toll-like receptor 4
NCBI Official Symbol
TLR4
NCBI Official Synonym Symbols
TOLL; CD284; TLR-4; ARMD10
NCBI Protein Information
toll-like receptor 4; hToll; homolog of Drosophila toll
UniProt Protein Name
Toll-like receptor 4
UniProt Gene Name
TLR4
UniProt Entry Name
TLR4_HUMAN

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Product Notes

The CD284 tlr4 (Catalog #AAA11902) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD284 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the CD284 tlr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD284, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.