Application Data
Mouse CD29 Monoclonal Antibody | anti-CD29 antibody
anti-human CD29-Purified Preservative Free
Gene Names
ITGB1; CD29
Reactivity
Human, Pig, Mouse
Applications
Flow Cytometry, Western Blot
Purity
Purified Preservative Free
Synonyms
CD29, Antibody; anti-human CD29-Purified Preservative Free; Monoclonal anti-human CD29 (ƒÒ1 integrin); anti-CD29 antibody
Host
Mouse
Reactivity
Human, Pig, Mouse
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
4B7R
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
135
Applicable Applications for anti-CD29 antibody
Flow Cytometry (FC), Western Blot (WB), FPS
Application Notes
FC Binding to Nalm-6 cells with GAM/FITC detection:
Specification: >1.0 log shift
Result: 1.3 log shift.
Specification: >1.0 log shift
Result: 1.3 log shift.
Performance
Five x 105 cultured human HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD29 antibody at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2° reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FC. Cells stained positive with a mean shift of 1.3 log 10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human ocular melanoma cell line, V+B2
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Application Data
Application Data
Related Product Information for anti-CD29 antibody
Information: Human CD29 is the beta subunit of an integrin family of molecules expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. CD29 can form heterodimer pairs with at least nine different alpha subunits. Antibody 4B7R recognizes the CD29 integrin subunit.
References
Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1612-1613.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,701 Da
NCBI Official Full Name
CD29, partial
NCBI Official Symbol
ITGB1
NCBI Official Synonym Symbols
CD29
NCBI Protein Information
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); integrin beta 1
UniProt Protein Name
CD29
UniProt Entry Name
A8CUR0_HORSE
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Product Notes
The CD29 (Catalog #AAA14855) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD29-Purified Preservative Free reacts with Human, Pig, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD29 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Western Blot (WB), FPS. FC Binding to Nalm-6 cells with GAM/FITC detection: Specification: >1.0 log shift Result: 1.3 log shift. Researchers should empirically determine the suitability of the CD29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD29, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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