Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody

Mouse anti Human CD3:FITC

Cat. Num. AAA50421

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant; >90 % by SDS PAGE
• Synonyms: CD3, Antibody; Mouse anti Human CD3:FITC; CD3 antibody | OKT3; anti-CD3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: OKT3
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant; >90 % by SDS PAGE
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.2mg/ml (varies by lot)

• Applicable Applications for anti-CD3 antibody: ELISA
• Immunogen: Human peripheral blood lymphocytes
• Conjugation: FITC
• Preparation and Storage: Store at +4 degree C. DO NOT FREEZE.; This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Application Data

(Surface staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE (Blue) with a relevant isotype control (Grey))

Application Data

(Surface staining of human peripheral blood lymphocytes with Mouse anti Human CD3:FITC (AAA50421) (Blue) with a relevant isotype control (Red))

Related Product Information for anti-CD3 antibody

Mouse anti Human CD3 antibody, clone OKT3, recognizes T-cell surface glycoprotein CD3 epsilon chain (CD3epsilon), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3epsilon is a subunit within the multiprotein cell surface receptor TCR-CD3 complex, which is expressed on the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009).

Mice that lack the CD3epsilon gene exhibit early arrest in T cell development and mutation of the gene in humans has been seen to result in severe combined immunodeficiency (SCID) (DeJarnette et al. 1998, de Saint Basile et al. 2004).

Mouse anti Human CD3, clone OKT3 is commonly used in flow cytometry in the phenotyping human T cells. Characterization of the OKT3 clone has shown that it is able to compete and block the binding of the CD3 clones SK7 and UCHT1 to human T cells (Li et al. 2005). The OKT3 clone has also been found to have immunosuppressive properties in vivo. As result of this, the clone was humanized to be used as a therapeutic drug called Muromonomab (Orthoclone). Muromonomab was used as an immunosuppressive including for after solid organ transplants, to prevent the activation of CD4- and CD8- T cells (Wilde & Goa 1996). However, the drug has since been discontinued due to the development of other treatments with similar efficacy and fewer side effects (Reichert 2012).

NCBI and Uniprot Product Information

• NCBI GI #: 1345708
• NCBI GeneID: 916
• UniProt Accession #: P07766; A8K997
• Molecular Weight: 207
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN