Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody

MOUSE ANTI HUMAN CD3

Cat. Num. AAA12296

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Functional Assay, Immunohistochemistry
• Purity: >90% by SDS PAGE. Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: CD3, Antibody; MOUSE ANTI HUMAN CD3; anti-CD3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: OKT3
• Purity/Purification: >90% by SDS PAGE. Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Purified IgG - liquid; ALEXA FLUOR 488, ALEXA FLUOR 647, ALEXA FLUOR 700, APC, FITC, Pacific Blue, Preservative Free, Purified, RPE, StarBright Blue 700, StarBright UltraViolet 400, StarBright UltraViolet 445, StarBright UltraViolet 510, StarBright Ultr
• Concentration: IgG concentration 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CD3 antibody: Flow Cytometry (FC/FACS), Functional Assay (FUNC), Immunohistochemistry-Frozen (IHC-F)
• Immunogen: Human peripheral blood lymphocytes
• Preparation and Storage: IgG concentration 0.5mg/ml

Application Data

(Figure A. StarBright Blue 810 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control. Figure B. StarBright Blue 810 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse anti Human CD3. All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data

(Figure A. StarBright Violet 570 conjugated Mouse anti Human CD4 and Alexa Fluor 488 conjugated Mouse IgG2a isotype control. Figure B. StarBright Violet 570 conjugated Mouse anti Human CD4 and Alexa Fluor 488 conjugated Mouse anti Human CD3. All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data

(Figure A. StarBright Violet 710 conjugated Mouse anti Human CD4 and Pacific Blue conjugated Mouse IgG2a isotype control . Figure B. StarBright Violet 710 conjugated Mouse anti Human CD4 and Pacific Blue conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data

(Figure A. StarBright Blue 580 conjugated Mouse anti Human CD4 and Alexa Fluor 700 conjugated Mouse IgG2a isotype control . Figure B. StarBright Blue 580 conjugated Mouse anti Human CD4 and Alexa Fluor 700 conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data

(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 and PE conjugated Mouse IgG2a isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 and PE conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data

(Figure A. StarBright Violet 790 conjugated Mouse anti Human CD4 and FITC conjugated Mouse IgG2a isotype control . Figure B. StarBright Violet 790 conjugated Mouse anti Human CD4 and FITC conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Related Product Information for anti-CD3 antibody

Mouse anti Human CD3 antibody, clone OKT3, recognizes T-cell surface glycoprotein CD3 epsilon chain (CD3epsilon), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3epsilon is a subunit within the multiprotein cell surface receptor TCR-CD3 complex, which is expressed on the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009). Mice that lack the CD3epsilon gene exhibit early arrest in T cell development and mutation of the gene in humans has been seen to result in severe combined immunodeficiency (SCID) (DeJarnette et al. 1998, de Saint Basile et al. 2004). Mouse anti Human CD3, clone OKT3 is commonly used in flow cytometry in the phenotyping human T cells. Characterization of the OKT3 clone has shown that it is able to compete and block the binding of the CD3 clones SK7 and UCHT1 to human T cells (Li et al. 2005). The OKT3 clone has also been found to have immunosuppressive properties in vivo. As result of this, the clone was humanized to be used as a therapeutic drug called Muromonomab (Orthoclone). Muromonomab was used as an immunosuppressive including for after solid organ transplants, to prevent the activation of CD4- and CD8- T cells (Wilde & Goa 1996). However, the drug has since been discontinued due to the development of other treatments with similar efficacy and fewer side effects (

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