Mouse CD3 Monoclonal Antibody | anti-CD3 antibody

MOUSE ANTI HUMAN CD3:RPE

Cat. Num. AAA12046

• Gene Names: CD3G; T3G; IMD17; CD3-GAMMA
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD3; Monoclonal Antibody; MOUSE ANTI HUMAN CD3:RPE; anti-CD3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: UCHT1
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 182

• Applicable Applications for anti-CD3 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of reconstitution.

Application Data

(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)

Application Data

(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Low power)

Application Data

(ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)

Application Data

(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3)

Application Data

(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)

NCBI and Uniprot Product Information

• NCBI GI #: 4557429
• NCBI GeneID: 917
• NCBI Accession #: NP_000064.1
• NCBI GenBank Nucleotide #: NM_000073.2
• Molecular Weight: 20,469 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 gamma chain
• NCBI Official Synonym Full Names: CD3g molecule, gamma (CD3-TCR complex)
• NCBI Official Symbol: CD3G
• NCBI Official Synonym Symbols: T3G; IMD17; CD3-GAMMA
• NCBI Protein Information: T-cell surface glycoprotein CD3 gamma chain; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 gamma chain
• UniProt Gene Name: CD3G
• UniProt Synonym Gene Names: T3G
• UniProt Entry Name: CD3G_HUMAN