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WB (Western Blot)
(Detection of mouse CD31 by western blot. Samples: Whole cell lysate (50 ug) from HEK293f and HEK293f overexpressing mouse CD31 prepared using NETN lysis buffer. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
CD31 recombinant antibody
Rabbit anti-CD31 Recombinant Monoclonal Antibody [BLR180J]
Gene Names
Pecam1; Cd31; Pecam; C85791; PECAM-1
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
CD31, Recombinant Antibody; Rabbit anti-CD31 Recombinant Monoclonal Antibody [BLR180J]; CD31/PECAM-1; Cd31; platelet endothelial cell adhesion molecule; Pecam; C85791; PECAM-1; CD antigen CD31; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1; CD31 recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Clone Number
BLR180J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for CD31 recombinant antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse CD31 by western blot. Samples: Whole cell lysate (50 ug) from HEK293f and HEK293f overexpressing mouse CD31 prepared using NETN lysis buffer. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse CD31 by western blot. Samples: Whole cell lysate (50 ug) from HEK293f and HEK293f overexpressing mouse CD31 prepared using NETN lysis buffer. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse CD31 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, Ba/F3, 70Z/3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody . Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse CD31 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, Ba/F3, 70Z/3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody . Lower Panel: Rabbit anti-COPB2 antibody .)
IHC (Immunohistochemisry)
(Detection of mouse CD31 in mouse uterus by IHC. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
IHC (Immunohistochemisry)
(Detection of mouse CD31 in mouse uterus by IHC. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse CD31 in mouse liver by IHC. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse CD31 in mouse liver by IHC. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
FCM/FACS (Flow Cytometry)
(Detection of mouse CD31 (shaded) in Ba/F3 cells by flow cytometry. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of mouse CD31 (shaded) in Ba/F3 cells by flow cytometry. Antibody: Rabbit anti-CD31 recombinant monoclonal antibody (AAA213629) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for CD31 recombinant antibody
CD31 is a cell adhesion molecule expressed mainly in platelets, endothelial cells, and a subset of leukocytes. CD31 localizes to cell-cell junctions and functions as a signaling molecule involved in a variety of vascular-related processes such as angiogenesis, platelet function, and inflammation.
Product Categories/Family for CD31 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80kDa; 76.2kDa
NCBI Official Full Name
platelet endothelial cell adhesion molecule isoform 1
NCBI Official Synonym Full Names
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol
Pecam1
NCBI Official Synonym Symbols
Cd31; Pecam; C85791; PECAM-1
NCBI Protein Information
platelet endothelial cell adhesion molecule
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
Pecam1
UniProt Synonym Gene Names
Pecam; Pecam-1; PECAM-1
UniProt Entry Name
PECA1_MOUSE
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Product Notes
The CD31 pecam1 (Catalog #AAA213629) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD31 Recombinant Monoclonal Antibody [BLR180J] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD31 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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