FCM/FACS (Flow Cytometry)
(Human peripheral blood monocytes were stained with anti-CD33 (clone: CD33 1C7/1) (filled histogram) or mouse IgG1 isotype control (open histogram).)
Mouse CD33 Monoclonal Antibody | anti-CD33 antibody
Anti-Human CD33, Biotin, (clone: CD33 1C7/1) (Mouse IgG1)
Gene Names
CD33; p67; SIGLEC3; SIGLEC-3
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Synonyms
CD33, Antibody; Anti-Human CD33, Biotin, (clone: CD33 1C7/1) (Mouse IgG1); CD33, Biotin, (clone: CD33 1C7/1) (Mouse IgG1); Anti-Human CD33 Monoclonal Antibody; anti-CD33 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
CD33 1C7/1
Specificity
CD33 (This antibody is specific for human CD33.)
Form/Format
Biotin
Concentration
0.1 mg/ml (varies by lot)
Sequence Length
364
Applicable Applications for anti-CD33 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Immunogen
CD33-Fc recombinant protein
Donor
BALB/c spleen
Fusion Partner
Sp2/0AG.14
Test Results
Tissue Distribution by Flow Cytometry Analysis;
Cell Concentration: 1x106 cells per test
Antibody Concentration Used: 0.1 ug/106 cells
Cell Concentration: 1x106 cells per test
Antibody Concentration Used: 0.1 ug/106 cells
Presentation
Biotin/FITC/PE conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Preparation and Storage
For all formats, store at 4°C. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC), aliquot and freeze unused portion at -20°C in volumes appropriate for single usage.
Avoid freeze/thaw cycle
Avoid freeze/thaw cycle
FCM/FACS (Flow Cytometry)
(Human peripheral blood monocytes were stained with anti-CD33 (clone: CD33 1C7/1) (filled histogram) or mouse IgG1 isotype control (open histogram).)
FCM/FACS (Flow Cytometry)
(Human peripheral blood monocytes were stained with anti-CD33 (clone: CD33 1C7/1) (filled histogram) or mouse IgG1 isotype control (open histogram).)
Related Product Information for anti-CD33 antibody
anti-Human CD33 monoclonal antibody specifically binds human CD33. CD33 is a 67 kDa transmembrane glycoprotein expressed on myeloid cells (granulocyte and macrophage precursors in the bone marrow). CD33 is part of the siglec (sialic acid-binding Ig-related lectins) family and shares common structural features to CD22 and Sialoadhesin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD33 antigen (gp67)
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
NCBI Protein Information
myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3
UniProt Protein Name
Myeloid cell surface antigen CD33
UniProt Gene Name
CD33
UniProt Synonym Gene Names
SIGLEC3; Siglec-3
UniProt Entry Name
CD33_HUMAN
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Product Notes
The CD33 cd33 (Catalog #AAA74221) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD33 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CD33 cd33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD33, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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