Application Data
(Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 Ab (ICO-115) (red) and isotype control Ab (green). PPI negative cell population was gated for analysis.)
Mouse CD34 Monoclonal Antibody | anti-CD34 antibody
CD34
Reactivity
Human and Rat. Others not known.
Applications
Flow Cytometry, Functional Assay
Purity
Purified Ab conjugated to PE
Synonyms
CD34, Antibody; CD34; Hematopoietic Progenitor Cell Antigen; HPCA1; Mucosialin; anti-CD34 antibody
Host
Mouse
Reactivity
Human and Rat. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
ICO-115
Specificity
This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
Purity/Purification
Purified Ab conjugated to PE
Form/Format
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM pbs with 0.05% BSA and 0.05% azide.
Sequence Length
385
Applicable Applications for anti-CD34 antibody
FCM/FACS (Flow Cytometry)
Localization
Cell surface
Positive Control
KG-1 cells, Tonsil, or Angiosarcoma
Human Chromosomal Location
1q32.2
Immunogen
Blast cells of a chronic myeloid leukemia patient
Quantity and Buffer
0.5 ml at 100ug/ml; Conjugated to PE
Preparation and Storage
Store at 2 to 8°C. Antibody is stable for 24 months.
Application Data
(Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 Ab (ICO-115) (red) and isotype control Ab (green). PPI negative cell population was gated for analysis.)
Application Data
(Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 Ab (ICO-115) (red) and isotype control Ab (green). PPI negative cell population was gated for analysis.)
References
1. Felshow DM et al. Blood 97:3768-3775 (2001).
2. Sato T et al. Blood 94:2548-2554 (1999).
2. Sato T et al. Blood 94:2548-2554 (1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90-110kDa
NCBI Official Full Name
hematopoietic progenitor cell antigen CD34 isoform a
NCBI Official Synonym Full Names
CD34 molecule
NCBI Official Symbol
CD34
NCBI Protein Information
hematopoietic progenitor cell antigen CD34; CD34 antigen
UniProt Protein Name
Hematopoietic progenitor cell antigen CD34
UniProt Gene Name
CD34
UniProt Entry Name
CD34_HUMAN
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Product Notes
The CD34 cd34 (Catalog #AAA62538) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD34 reacts with Human and Rat. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's CD34 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD34 cd34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD34, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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