CD38 recombinant antibody

Rabbit anti-CD38 Recombinant Monoclonal Antibody [BLR123H]

Cat. Num. AAA23840

• Gene Names: CD38; ADPRC1; ADPRC 1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: CD38, Recombinant Antibody; Rabbit anti-CD38 Recombinant Monoclonal Antibody [BLR123H]; CD antigen CD38; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; NAD(+) nucleosidase; ecto-nicotinamide adenine dinucleotide glycohydrolase; T10; cyclic ADP-ribose hydrolase 1; cluster of differentiation 38; CD38 antigen (p45); cADPr hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase 1; ADPRC1; 2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-ADP-ribosyl cyclase; ADPRC 1; CD38 molecule; CD38 recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: BLR123H
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 50ug/ml (varies by lot)

• Applicable Applications for CD38 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Residues 43-300 (ECD)
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human CD38 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, MOLT-4, RPMI-8226, CCRF-CEM, A-549, Ramos, Raji, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human CD38 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 lot 1) used for IP at 20 ul/mg lysate. CD38 was also immunoprecipitated by rabbit anti-CD38 antibody BL1874B-2A3. For blotting immunoprecipitated CD38, AAA23840 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)

IHC (Immunohistochemistry)

(Detection of human CD38 in FFPE colon carcinoma by immunohistochemistry. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human CD38 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human CD38 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody AAA23840 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human CD38 in FFPE CCRF-CEM cells by immunocytochemistry. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human CD38 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-CD38 recombinant monoclonal (AAA23840) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for CD38 recombinant antibody

CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1) is a type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. The functional CD38 molecule is a dimer, with the central portion containing the catalytic site. CD38 is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [taken from NCBI Entrez Gene (Gene ID: 952)].

Product Categories/Family for CD38 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 38454326
• NCBI GeneID: 952
• NCBI Accession #: NP_001766.2
• NCBI GenBank Nucleotide #: NM_001775.3
• UniProt Accession #: P28907; O00121; O00122; Q96HY4
• Molecular Weight: 13,788 Da
• NCBI Official Full Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: ADPRC1; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1
• UniProt Entry Name: CD38_HUMAN