Mouse anti-Human CD38 Antibody | anti-CD38 antibody

Anti-Hu CD38 Purified

Cat. Num. AAA129350

• Gene Names: CD38; T10; ADPRC 1
• Reactivity: Human
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
• Purity: Purified by protein-A affinity chromatography.
• Synonyms: CD38; N/A; Anti-Hu CD38 Purified; CD38 molecule; ADPRC1, cADPr hydrolase 1, T10, NAD(+) nucleosidase, ADP-ribosyl cyclase 1; anti-CD38 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: HB7
• Specificity: The mouse monoclonal antibody HB7 (HB-7) recognizes an extracellular epitope within amino acids 273-285 of human CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab.
• Purity/Purification: Purified by protein-A affinity chromatography.
• Form/Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD38 antibody: IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: BJAB cell line
• Conjugation: Purified
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human monocytes (red-filled) from CD19 negative CD38 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD38 (HB7) purified antibody (concentration in sample 1.67ug/ml, GAM APC).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD38 (HB7) purified antibody (concentration in sample 1.67ug/ml, GAM APC) and anti-human CD19 (LT19) PE antibody (10ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD38 (HB7) purified antibody (concentration in sample 1.67ug/ml, GAM APC).)

Related Product Information for anti-CD38 antibody

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration. CD38 belongs to markers of B cell subsets, leukemia, and a target of immunotherapy in myeloma treatment.

References

*Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP-ribose-CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase-catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1;358(Pt 2):399-406., URL: https://www.ncbi.nlm.nih.gov/pubmed/11513738?ordin,*Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism. Int Immunol. 2006 Jul;18(7):1029-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/16720618?ordin,*Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol. 2007 Dec 1;179(11):7827-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/18025229?ordin,
*Goldmacher VS, Bourret LA, Levine BA, Rasmussen RA, Pourshadi M, Lambert JM, Anderson KC: Anti-CD38-blocked ricin: an immunotoxin for the treatment of multiple myeloma. Blood 1994 Nov 1;84(9):3017-25., URL: https://pubmed.ncbi.nlm.nih.gov/7524764/,*Zhao YJ, Zhu WJ, Wang XW, Zhang LH, Lee HC: Determinants of the membrane orientation of a calcium signaling enzyme CD38. Biochim Biophys Acta 2015 Sep;1853(9):2095-103., URL: https://pubmed.ncbi.nlm.nih.gov/25447548/,*Berthelier V, Laboureau J, Boulla G, Schuber F, Deterre P: Probing ligand-induced conformational changes of human CD38. Eur J Biochem. 2000 May;267(10):3056-64., URL: https://pubmed.ncbi.nlm.nih.gov/10806406/,*Li T, Qi S, Unger M, Hou YN, Deng QW, Liu J, Lam CM, Wang XW, Xin D, Zhang P, Koch-Nolte F, Hao Q, Zhang H, Lee HC, Zhao YJ: Immuno-targeting the multifunctional CD38 using nanobody. Sci Rep. 2016 Jun 2;6:27055. , URL: https://pubmed.ncbi.nlm.nih.gov/27251573/,*Ghose J, Viola D, Terrazas C, Caserta E, Troadec E, Khalife J, Gunes EG, Sanchez J, McDonald T, Marcucci G, Kaur B, Rosenzweig M, Keats J, Rosen S, Krishnan A, Satoskar AR, Hofmeister CC, Pichiorri F: Daratumumab induces CD38 internalization and impairs myeloma cell adhesion. Oncoimmunology. 2018 Jul 23;7(10):e1486948., URL: https://pubmed.ncbi.nlm.nih.gov/30288349/,*Ferrero E, Orciani M, Vacca P, Ortolan E, Crovella S, Titti F, Saccucci F, Malavasi F: Characterization and phylogenetic epitope mapping of CD38 ADPR cyclase in the cynomolgus macaque. BMC Immunol. 2004 Sep 21;5:21., URL: https://pubmed.ncbi.nlm.nih.gov/15383153/,*Ratuszny D, Skripuletz T, Wegner F, Groß M, Falk C, Jacobs R, Ruschulte H, Stangel M, Suhs KW: Case report: Daratumumab in a patient with severe refractory anti-NMDA receptor encephalitis. Front Neurol. 2020 Dec 22;11:602102., URL: https://pubmed.ncbi.nlm.nih.gov/33414761/,*Verhoeven D, Grinwis L, Marsman C, Jansen MH, van Leeuwen E, Kuijpers TW: B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses. Life Sci Alliance 2023 Jul 7;6(9):e202302214., URL: https://pubmed.ncbi.nlm.nih.gov/37419630/,*Shrestha P, Astter Y, Davis DA, Zhou T, Yuan CM, Ramaswami R, Wang HW, Lurain K, Yarchoan R: Daratumumab induces cell-mediated cytotoxicity of primary effusion lymphoma and is active against refractory disease. Oncoimmunology 2023 Jan 7;12(1):2163784., URL: https://pubmed.ncbi.nlm.nih.gov/36632565/,

NCBI and Uniprot Product Information

• NCBI GI #: 55977782
• NCBI GeneID: 952
• UniProt Accession #: P28907; O00121; O00122; Q96HY4
• Molecular Weight: 300
• NCBI Official Full Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: T10; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; CD38 antigen (p45); NAD(+) nucleosidase; ADP-ribosyl cyclase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cycli
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1
• UniProt Entry Name: CD38_HUMAN