CD3E recombinant antibody

Rabbit anti-CD3E Recombinant Monoclonal Antibody [BLR174J]

Cat. Num. AAA23851

• Gene Names: Cd3e; CD3; T3e; AI504783; CD3epsilon
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: CD3E; Monoclonal Recombinant Antibody; Rabbit anti-CD3E Recombinant Monoclonal Antibody [BLR174J]; AI504783; CD antigen CD3e; CD3; CD3epsilon; T3e; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3 antigen; epsilon polypeptide; CD3E recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR174J
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 500ug/ml (varies by lot)

• Applicable Applications for CD3E recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 12 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Between 139 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse CD3E by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, NIH 3T3, TCMK-1, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of human CD3E by western blot. Samples: Whole cell lysate (10 ug) from K-562, Jurkat, SK-MEL-28, MOLT-4, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation)

(Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1) used for IP at 12 ul/mg lysate. CD3E was also immunoprecipitated by a second antibody against a different epitope of CD3E . For blotting immunoprecipitated CD3E, AAA23851 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistochemistry)

(Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of mouse CD3E in FFPE mouse spleen by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of mouse CD3E in FFPE mouse CTLL-2 cells by ICC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human CD3E in FFPE MOLT-4 cells by ICC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human CD3 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lo t1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for CD3E recombinant antibody

CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma,-delta and-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The epsilon polypeptide plays an essential role in T-cell development [taken from NCBI Entrez Gene (Gene ID: 916)].

Product Categories/Family for CD3E recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 6680886
• NCBI GeneID: 12501
• NCBI Accession #: NP_031674.1
• NCBI GenBank Nucleotide #: NM_007648.4
• UniProt Accession #: P22646
• Molecular Weight: 21,393 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3 antigen, epsilon polypeptide
• NCBI Official Symbol: Cd3e
• NCBI Official Synonym Symbols: CD3; T3e; AI504783; CD3epsilon
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: Cd3e
• UniProt Entry Name: CD3E_MOUSE