Rabbit anti-Pig CD3e Antibody | anti-CD3e antibody

Anti-CD3e [898H2-6-15], Rabbit IgG

Cat. Num. AAA72253

• Gene Names: CD3E; CD3
• Reactivity: Pig
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: CD3e; N/A; Anti-CD3e [898H2-6-15], Rabbit IgG; Anti-CD3e [898H2-6-15]; Leu4/T3; Cluster of Differentiation 3 epsilon chain; T cell glycoprotein CD3 epsilon chain; anti-CD3e antibody

• Host: Rabbit
• Reactivity: Pig
• Isotype: Rabbit IgG, Kappa
• Clone Number: 898H2-6-15
• Specificity: Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3, and the e chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD3e antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
• Immunogen: Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.
• Origin Pub PMID: 21866954
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow cytometry using the Anti-CD3e antibody 898H2-6-15 (AAA72253). Paraformaldehyde fixed pig peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 898H2-6-15 (AAA72253-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

WB (Western Blot)

(Western Blot using Anti-CD3e antibody 898H2-6-15 (AAA72253). Pig spleen(A) (1ug/ml), pig thymus(B) (0.1ug/ml) and lymph node(C) (0.1ug/ml) tissue lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 898H2-6-15 (AAA72253-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)

IF (Immunofluorescence)

(Immunofluorescence staining of pig peripheral blood monocytes with anti-CD3e (AAA72253) 898H2-6-15 Immunofluorescence analysis of paraformaldehyde fixed pig peripheral blood monocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of 898H2-6-15 (AAA72253-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72253-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-CD3e antibody

The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor-associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an 'immunotoxin' has been shown to deplete circulating T cells in swine without observation of any clinical toxicities.

Product Categories/Family for anti-CD3e antibody

Lymphocytes; xenotransplantation; grafting

References

Huang et al. Characterization of a monoclonal anti-porcine CD3 antibody Xenotransplantation 1999: 5: 201-212 PMID:10503787

NCBI and Uniprot Product Information

• NCBI GI #: 78099944
• NCBI GeneID: 397455
• UniProt Accession #: Q7YRN2
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: CD3
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Entry Name: CD3E_PIG