Hamster anti-Mouse CD3z Monoclonal Antibody | anti-CD3z antibody
Anti-Mouse CD3z, FITC (Clone H146-968) (hamster IgG)
Gene Names
CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
Reactivity
Mouse
Applications
Immunofluorescence, Immunohistochemistry
Synonyms
CD3z, Antibody; Anti-Mouse CD3z, FITC (Clone H146-968) (hamster IgG); CD3z, FITC (Clone H146-968) (hamster IgG); FITC Anti-Mouse CD3z (CD247) Monoclonal Antibody; anti-CD3z antibody
Host
Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Hamster IgG2k
Clone Number
H146-968
Specificity
CD3z (CD247) (Mouse CD3z (zeta chain); also known as CD247)
Form/Format
FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
Concentration
Antibody Concentration: 0.1 mg/ml (varies by lot)
Sequence Length
164
Applicable Applications for anti-CD3z antibody
IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
KLH conjugated mouse zeta-chain (CD3) sequence 151-164
Donor
Armenian hamster
Presentation
100 ug or 300 ug FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Preparation and Storage
Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles.
Related Product Information for anti-CD3z antibody
FITC Anti-Mouse CD3z Monoclonal Antibody detects the zeta subunit of the T cell receptor (TCR). The TCR complex consists of a ligand specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma, delta, eta and zeta subunits. This complex regulates assembly and expression of the receptor and is thought to be responsible for transmembrane transduction of signals after binding to TCRalphabeta. Studies have suggested that the CD3z subunit (also know as CD247) plays an important role in two distinct cellular compartments. In the cytoplasm, it may function to regulate Ag receptor expression and in the plasma membrane it may be required for optimal signaling by the physiologic ligand Ag/MHC. The CD3z subunit is also associated with the CD16 IgG Fc receptor on NK cells. This clone (H146-968) has been reported to work in flow cytometry (in permeabilized cells), Western blotting, and ELISA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,696 Da
NCBI Official Full Name
CD3Z antigen, zeta polypeptide (TiT3 complex)
NCBI Official Synonym Full Names
CD247 molecule
NCBI Official Symbol
CD247
NCBI Official Synonym Symbols
T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
NCBI Protein Information
T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3
UniProt Protein Name
T-cell surface glycoprotein CD3 zeta chain
UniProt Gene Name
CD247
UniProt Synonym Gene Names
CD3Z; T3Z; TCRZ
UniProt Entry Name
CD3Z_HUMAN
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Product Notes
The CD3z cd247 (Catalog #AAA74142) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD3z, FITC (Clone H146-968) (hamster IgG) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3z can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD3z cd247 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3z, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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