FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CD4 expression on human peripheral blood lymphocytes. Cells were stained with PE-conjugated anti-Human CD4 and FITC conjugated anti-Human CD3 (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.Flow Cytometry was perfomed on a BD FACSCalibur flow cytometry system.)
Mouse CD4 Monoclonal Antibody | anti-CD4 antibody
Anti-CD4 Antibody (PE), Mouse Monoclonal
Gene Names
CD4; CD4mut
Applications
Flow Cytometry, Functional Assay
Synonyms
CD4, Antibody; Anti-CD4 Antibody (PE), Mouse Monoclonal; CD4 Antibody (PE), Mouse MAb; CD4 molecule; Anti-CD4mut Antibody; anti-CD4 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
08
Specificity
Human CD4
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
1ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD4 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Recombinant Human CD4 protein
Conjugation
PE
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD4 (rh CD4) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CD4 expression on human peripheral blood lymphocytes. Cells were stained with PE-conjugated anti-Human CD4 and FITC conjugated anti-Human CD3 (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.Flow Cytometry was perfomed on a BD FACSCalibur flow cytometry system.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CD4 expression on human peripheral blood lymphocytes. Cells were stained with PE-conjugated anti-Human CD4 and FITC conjugated anti-Human CD3 (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.Flow Cytometry was perfomed on a BD FACSCalibur flow cytometry system.)
Product Categories/Family for anti-CD4 antibody
References
Farrar WL, et al. (1988) Characterization of CD4 glycoprotein determinant-HIV envelope protein interactions: perspectives for analog and vaccine development. Crit Rev Immunol. 8(4): 315-39.Biddison WE, et al. (1989) CD4 expression and function in HLA class II-specific T cells. Immunol Rev. 109: 5-15.Singh SK, et al. (2012) Mapping the interaction between the cytoplasmic domains of HIV-1 viral protein U and human CD4 with NMR spectroscopy. FEBS J. 279(19):3705-14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD4 isoform 1
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
CD4
NCBI Official Synonym Symbols
CD4mut
NCBI Protein Information
T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Gene Name
CD4
UniProt Entry Name
CD4_HUMAN
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Product Notes
The CD4 cd4 (Catalog #AAA254899) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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