Application Data
(Published customer image: Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)
Mouse CD4 Monoclonal Antibody | anti-CD4 antibody
MOUSE ANTI SHEEP CD4:RPE
Perservative Stabilisers
Fusion Partners
Shelf Life: 12 months from date of reconstitution.
Application Data
(Published customer image: Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)
Application Data
(Published customer image: Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)
Application Data
(Staining of ovine peripheral blood lymphocytes with Mouse anti Ovine CD4 Alexa Fluor 647)
Application Data
(Staining of ovine peripheral blood lymphocytes with Mouse anti Ovine CD4 Alexa Fluor 647)
Application Data
(Published customer image: Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of ?d T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)
Application Data
(Published customer image: Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of ?d T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.)
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Product Notes
The CD4 cd4 (Catalog #AAA49647) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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