Mouse anti-Human CD4 Monoclonal Antibody | anti-CD4 antibody

Mouse Monoclonal Antibody (Mab)

Cat. Num. AAA283569

• Gene Names: CD4; CD4mut
• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: CD4, Antibody; Mouse Monoclonal Antibody (Mab); T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4; anti-CD4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: OKT4
• Specificity: This CD4 monoclonal antibody is generated from mouse immunized with CD4 recombinant protein.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.47mg/ml (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-CD4 antibody: ELISA, WB (Western Blot)
• Antigen Source: HUMAN
• Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
• Cellular Location: Cell membrane; Single-pass type I membrane protein Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(CD4 Antibody western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CD4 antibody detected the CD4 protein (arrow).)

Related Product Information for anti-CD4 antibody

This gene encodes a membrane glycoprotein of T lymphocytes
that interacts with major histocompatibility complex class II
antigenes and is also a receptor for the human immunodeficiency
virus. This gene is expressed not only in T lymphocytes, but also
in B cells, macrophages, and granulocytes. It is also expressed in
specific regions of the brain. The protein functions to initiate or
augment the early phase of T-cell activation, and may function as
an important mediator of indirect neuronal damage in infectious and
immune-mediated diseases of the central nervous system. Multiple
alternatively spliced transcript variants encoding different
isoforms have been identified in this gene.

Product Categories/Family for anti-CD4 antibody

Immunology; Signal Transduction

References

Pourgheysari, B., et al. Blood 116(16):2968-2974(2010)
Rudd, C.E., et al. J. Immunol. 185(5):2645-2649(2010)
Ammirati, E., et al. Arterioscler. Thromb. Vasc. Biol. 30(9):1832-1841(2010)
Schenkel, J.M., et al. J. Immunol. 185(4):2013-2019(2010)
Lee, K.M., et al. Am. J. Hematol. 85(8):560-563(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4
• UniProt Protein Name: T-cell surface glycoprotein CD4
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN