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Application Data
(Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody , red in A and Mouse anti Rat CD8 , green in B. C is merged image with nuclear counterstaining with DAPI in blue. High power)
Mouse CD4 Monoclonal Antibody | anti-CD4 antibody
MOUSE ANTI RAT CD4 (DOMAIN 2)
Gene Names
Cd4; p55; W3/25
Applications
Immunoprecipitation, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD4, Antibody; MOUSE ANTI RAT CD4 (DOMAIN 2); anti-CD4 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
OX-35
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
457
Applicable Applications for anti-CD4 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody , red in A and Mouse anti Rat CD8 , green in B. C is merged image with nuclear counterstaining with DAPI in blue. High power)
Application Data
(Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody , red in A and Mouse anti Rat CD8 , green in B. C is merged image with nuclear counterstaining with DAPI in blue. High power)
Application Data
(Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody , red in A and Mouse anti Rat CD8 , green in B. C is merged image with nuclear counterstaining with DAPI in blue. Low power)
Application Data
(Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody , red in A and Mouse anti Rat CD8 , green in B. C is merged image with nuclear counterstaining with DAPI in blue. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
Related Product Information for anti-CD4 antibody
Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes the rat CD4 cell surface antigen, a ~55kDa glycoprotein expressed by helper T cells and weakly by monocytes. This antibody recognizes a different epitope on the CD4 molecule than the W3/25 monoclonal Mouse anti Rat CD4 antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,438 Da
NCBI Official Full Name
T-cell surface glycoprotein CD4
NCBI Official Synonym Full Names
Cd4 molecule
NCBI Official Symbol
Cd4
NCBI Official Synonym Symbols
p55; W3/25
NCBI Protein Information
T-cell surface glycoprotein CD4; CD4 antigen; T-cell surface antigen T4/Leu-3; W3/25 antigen
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Gene Name
Cd4
UniProt Entry Name
CD4_RAT
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Product Notes
The CD4 cd4 (Catalog #AAA49268) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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