Application Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
Rat anti-Mouse CD4 Monoclonal Antibody | anti-CD4 antibody
Anti-Mouse CD4, Purified, Low Endotoxin (Clone YTS 191.1)(rat IgG2b)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
CD4, Antibody; Anti-Mouse CD4, Purified, Low Endotoxin (Clone YTS 191.1)(rat IgG2b); anti-CD4 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
YTS 191.1.2
Specificity
Mouse CD4 (L3/T4; Ly4)
Purity/Purification
Purified
Form/Format
Low Endotoxin
Applicable Applications for anti-CD4 antibody
FCM/FACS (Flow Cytometry)
Conjugation
Unconjugated
Preparation and Storage
Store at 4 degree C.
Application Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
Application Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
FCM/FACS (Flow Cytometry)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
FCM/FACS (Flow Cytometry)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
Related Product Information for anti-CD4 antibody
The CD4 (L3/T4) antigen is thought to be the murine equivalent of the human Leu3/OKT4 antigen. This T cell surface molecule appears to be expressed by the helper/inducer subset of murine T cells and by delayed hypersensitivity T cells but not by cytotoxic T cells or their precursors. CD4 (L3/T4) and CD8a (Ly 2) have been shown to be present on mutually exclusive T cells in the peripheral lymphoid organs but the thymus contains cells expressing both CD4 (L3/T4) and CD8a (Ly 2).
anti-mouse CD4 (L3/T4) mAb binds to approximately 85% of mouse thymocytes, 20% splenocytes, 50% lymph node cells, and a small number of bone marrow cells. It detects a protein of approximately 52 kDa on SDS-PAGE "Western Blots" (from Con A blast cell membranes) and is therefore similar to a well characterized human Leu3/T4 antigen (4).
anti-mouse CD4 (L3/T4) mAb binds to approximately 85% of mouse thymocytes, 20% splenocytes, 50% lymph node cells, and a small number of bone marrow cells. It detects a protein of approximately 52 kDa on SDS-PAGE "Western Blots" (from Con A blast cell membranes) and is therefore similar to a well characterized human Leu3/T4 antigen (4).
References
1) Cobbald S.P., Jayasuriya A., Nash A., Prospero T.D., & Waldmann H., (1984) Nature. Therapy with monoclonal antibodies by elimination of T cell subsets in vivo . 312, 5994, 548-551.
2) Cobbald S.P., Martin G., Lovat P.C., & Waldmann H., 8th International Conference on Lymphatic Tissues and Germinal Centres. Plenum Press (Ed. Klaus G.) in press (1984) Immunosuppression with monoclonal antibodies - rules for effective serotherapy.
3) Agel N.M., Clark M., Cobbald S.P. & Waldmann H. (1984) J. Immunol. Methods. Immunohistological Screening in the selection of monoclonal antibodies: the use of isotype specific antiglobulins. 69, 207-214.
4) Dialynas D.P., et al. (1983) J. of Immunol. Characterization of the murine T cell surface antigen designated L3/T4, identified by monoclonal antibody GK1.5. Similarity of L3/T4 to human Leu3/T4 molecule. U131U, 2445-2451.
5) Mueller, R. et al. (1997) J. of Immunol. 159: 1599-1603. IL-4 Expression by Grafts from Transgenic Mice Fails to Prevent Allograft Rejection.
2) Cobbald S.P., Martin G., Lovat P.C., & Waldmann H., 8th International Conference on Lymphatic Tissues and Germinal Centres. Plenum Press (Ed. Klaus G.) in press (1984) Immunosuppression with monoclonal antibodies - rules for effective serotherapy.
3) Agel N.M., Clark M., Cobbald S.P. & Waldmann H. (1984) J. Immunol. Methods. Immunohistological Screening in the selection of monoclonal antibodies: the use of isotype specific antiglobulins. 69, 207-214.
4) Dialynas D.P., et al. (1983) J. of Immunol. Characterization of the murine T cell surface antigen designated L3/T4, identified by monoclonal antibody GK1.5. Similarity of L3/T4 to human Leu3/T4 molecule. U131U, 2445-2451.
5) Mueller, R. et al. (1997) J. of Immunol. 159: 1599-1603. IL-4 Expression by Grafts from Transgenic Mice Fails to Prevent Allograft Rejection.
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Product Notes
The CD4 (Catalog #AAA74286) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD4, Purified, Low Endotoxin (Clone YTS 191.1)(rat IgG2b) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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