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Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40: RPE)
Rat CD40 Monoclonal Antibody | anti-CD40 antibody
RAT ANTI MOUSE CD40:Low Endotoxin
Gene Names
Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Functional Assay
Synonyms
CD40, Antibody; RAT ANTI MOUSE CD40:Low Endotoxin; anti-CD40 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3/23
Form/Format
Low Endotoxin
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1 mg/ml (varies by lot)
Sequence Length
260
Applicable Applications for anti-CD40 antibody
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/50
Flow Cytometry: Maximum Dilution: 1/50
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Extracellular domain of Mouse CD40 and the Fc portion of Human IgG1
Fusion Partners
Fusion Partners
Target Species
Mouse
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40: RPE)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40: RPE)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40:FITC)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40:FITC)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40:RPE)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40:RPE)
Application Data
(Staining of mouse splenocytes cells with Rat anti Mouse CD40)
Application Data
(Staining of mouse splenocytes cells with Rat anti Mouse CD40)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40: Alexa Fluor 647)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40: Alexa Fluor 647)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40: Low Endotoxin)
Application Data
(Staining of mouse splenocytes with Rat anti Mouse CD40: Low Endotoxin)
Related Product Information for anti-CD40 antibody
Rat anti Mouse CD40 antibody, clone 3/23 recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity of Rat anti Mouse CD40 antibody, clone 3/23 was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40. Rat anti Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,878 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 antigen
NCBI Official Symbol
Cd40
NCBI Official Synonym Symbols
IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; CD40L receptor; T-cell differentiation antigen; tumor necrosis factor receptor superfamily, member 5
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
Cd40
UniProt Synonym Gene Names
Tnfrsf5
UniProt Entry Name
TNR5_MOUSE
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Product Notes
The CD40 cd40 (Catalog #AAA11897) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD40 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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